Table 4 Results obtained by applying the proposed HPTLC-densitometry method and CZE method for the determination of amiloride hydrochloride, timolol maleate and hydrochlorothiazide in moducren® tablets and application of standard addition technique
Pharmaceutical formulation | HPTLC-densitometry | CZE-DAD method | |||||||
|---|---|---|---|---|---|---|---|---|---|
Drug | %Found ± SDa | Standard addition technique | %Found ± SDa | Standard addition technique | |||||
Claimed (µg/band) | Pure added (µg/band) | %R of the pure addedb | Claimed (μg/mL) | Pure added (μg/mL) | %R of the pure added amountb | ||||
Moducren® tablets B.N. AJC 066 (each tablet is labelled to contain 2.5 mg AML, 10 mg TIM and 25 mg HCT) | AML | 97.96 ± 1.25 | 1.0 | 0.5 | 97.20 | 98.34 ± 1.10 | 40.0 | 20.0 | 98.95 |
1.0 | 98.61 | 40.0 | 100.17 | ||||||
2.0 | 99.98 | 80.0 | 100.22 | ||||||
Mean ± SD | 98.59 ± 1.39 | Mean ± SD | 99.78 ± 0.72 | ||||||
HCT | 99.76 ± 0.99 | 5.0 | 2.5 | 97.53 | 100.47 ± 1.18 | 50.0 | 25.0 | 100.69 | |
5.0 | 100.29 | 50.0 | 101.11 | ||||||
10.0 | 99.78 | 100.0 | 99.26 | ||||||
Mean ± SD | 99.20 ± 1.47 | Mean ± SD | 100.35 ± 0.97 | ||||||
TIM | 100.77 ± 1.10 | 4.0 | 2.0 | 100.43 | 99.26 ± 1.02 | 40.0 | 20.0 | 100.13 | |
4.0 | 99.52 | 40.0 | 98.40 | ||||||
8.0 | 101.43 | 80.0 | 99.79 | ||||||
Mean ± SD | 99.20 ± 1.47 | Mean ± SD | 99.44 ± 0.92 | ||||||