Table 2 Regression and validation parameters of the proposed HPTLC-densitometry and CZE-DAD method

Range (µg/band for HPTLC, and µg/mL for CZE)

0.5–10.0

1.0–16.0

1.0–14.0

0.05–1.0

0.05–1.0

20.0–160.0

10.0–200.0

10.0–120.0

10.0–100.0

Slope (b)^a

2160.7

–

2303.3

–

–

0.1696

0.1993

0.0923

0.2814

Coefficient 1 (b1)^b

–

 − 85.058

–

 − 11,534

 − 1790.9

–

–

–

–

Coefficient 2 (b2)^b

–

3363.9

–

25,358

6175.2

–

–

–

–

Intercept (a)^{a, b}

1793.8

10,404

5197

 − 478.24

161.03

1.8958

3.2617

0.6533

0.5594

Correlation Coefficient (r)

0.9999

0.9998

0.9998

0.9998

0.9998

0.9997

0.9998

0.9997

0.9996

Accuracy (Mean ± SD)

100.81 ± 0.89

99.90 ± 1.59

100.10 ± 1.39

99.44 ± 1.36

100.53 ± 1.02

100.16 ± 1.65

99.67 ± 1.13

99.99 ± 1.75

100.02 ± 1.21

Precision

(± %RSD)^c

0.80

0.47

0.68

0.64

0.30

0.88

1.29

0.62

0.99

(± %RSD)^d

1.01

1.44

0.81

1.63

1.03

1.10

1.64

1.43

1.74

LOD^e (µg/band for HPTLC, and µg/mL for CZE)

–

–

–

0.01

0.01

–

–

–

2.94

LOQ^e (µg/band for HPTLC, and µg/mL for CZE)

–

–

–

0.03

0.02

–

–

–

8.92

Robustness^f

1.21

1.43

1.11

1.31

0.98

1.76

1.45

1.78

1.53