Table 6 Accuracy of the proposed HPLC method using mobile phase n-hexane: ethanol: DEA (75: 25: 0.1 by volume) for quantitative determination of metoprolol and nebivolol enantiomers
Metoprolol | Nebivolol | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Taken \(\mathrm{\mu g}/\mathrm{mL}\) | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.1 | Recovery % enant.1 | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.2 | Recovery % enant.2 | Taken \(\mathrm{\mu g}/\mathrm{mL}\) | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.1 | Recovery % enant.1 | Found \(\mathrm{\mu g}/\mathrm{mL}\) enant.2 | Recovery % enant.2 |
50.00 | 49.71 | 99.42 | 50.30 | 100.60 | 50.00 | 49.52 | 99.04 | 50.47 | 100.94 |
100.00 | 100.18 | 100.18 | 99.74 | 99.74 | 100.00 | 100.26 | 100.26 | 101.24 | 101.24 |
150.00 | 150.44 | 100.29 | 150.58 | 100.39 | 150.00 | 150.51 | 100.34 | 152.41 | 101.61 |
180.00 | 177.29 | 98.49 | 179.81 | 99.89 | 180.00 | 180.19 | 100.11 | 178.59 | 99.22 |
200.00 | 203.65 | 101.83 | 203.77 | 101.89 | 200.00 | 201.17 | 100.59 | 200.79 | 100.40 |
220.00 | 219.84 | 99.93 | 218.36 | 99.25 | 220.00 | 219.41 | 99.73 | 219.24 | 99.65 |
250.00 | 248.91 | 99.56 | 249.21 | 99.68 | 250.00 | 248.08 | 99.23 | 249.29 | 99.72 |
Mean | 99.96 | 100.21 | 99.90 | 100.40 | |||||
R.S.D | 1.022 | 0.867 | 0.587 | 0.898 | |||||