Enantioseparation, quantification, molecular docking and molecular dynamics study of five β-adrenergic blockers on Lux-Cellulose-2 column

Table 3 Regression and validation parameters of the proposed HPLC method for quantitative determination of bisoprolol, carvedilol and atenolol enantiomers using mobile phase n-hexane: ethanol: DEA (60: 40: 0.1 by volume)

Parameters	Bisoprolol		Carvedilol		Atenolol
Parameters	Enant. 1	Enant.2	Enant. 1	Enant.2	Enant. 1	Enant.2
Linearity
Slope(Au-Sec.ml/µg)	3.2975	3.3319	11.8970	11.8030	4.0228	3.9870
Intercept (Au-Sec)	− 1.2267	− 1.1400	− 15.9670	− 9.9133	50.3600	51.4800
Correlation coefficient r	0.9995	0.9995	0.9996	0.9996	0.9995	0.9995
Range (\(\upmu \)g/mL)	50–300	50–300	20–120	20–120	50–300	50–300
Accuracy (Mean + R.S.D.%)	99.93 + 0.942	99.93 + 0.432	99.98 + 1.030	100.09 + 0.956	100.35 + 0.849	99.97 + 1.185
*Robustness (RSD %)	0.821	0.426	0.962	0.843	0.787	0.982
**Robustness (RSD %)	0.624	0.408	0.773	0.641	0.691	0.522
Precision (RSD %)
Repeatabilty^a	0.531	0.314	0.732	0.548	0.492	0.821
Reproducibility^b	0.722	0.409	0.811	0.699	0.588	0.911
LOD^c (\(\upmu \)g/mL)	6.7	6.7	2.7	2.7	6.7	6.7
LOQ^c (\(\upmu \)g/mL)	20	20	8	8	20	20

^aIntra-day (n = 3), average of three concentration of each drug repeated three times within the same day
^bInter-day (n = 3), average of three concentration of each drug repeated three times three consecutive days
^cLOD = 3.3 (SD/S), LOQ = 10 (SD/S), where SD is the standard deviation of regression residuals and S is the slope of calibration curves
*The robustness of the proposed method was investigated by analysis of samples under deliberate change in flow rate ± 0.1 mL/min
**The robustness of the proposed method was investigated by analysis of samples under deliberate change in Temperature ± 0.1 °C

ISSN: 2661-801X