Table 1 Gradient elution conditions for HPLC fingerprinting of Shenmai injection
From: Quality monitoring of Shenmai injection by HPLC pharmacodynamic fingerprinting
Time (min) | Solution A: acetonitrile (%) | Solution B: water (%) |
|---|---|---|
0 | 0 | 100 |
20 | 5 | 95 |
30 | 20 | 80 |
45 | 28 | 72 |
75 | 60 | 40 |
80 | 90 | 10 |
90 | 90 | 10 |