Analyte | Method code | Description |
---|---|---|
Arsenic Cadmium Chromium Lead Nickel Selenium Beryllium | Eurofins: EN ISO 17294–2:2016/EN 13805:2014 | Digestion was performed in a microwave oven with a mix of nitric acid, hydrochloric acid, and hydrogen peroxide, followed by detection and quantification on an inductively coupled plasma mass spectrometry (ICP-MS) |
Mercury | Eurofins: EN 16277:2012 | Digestion was performed according to Annex D of EN16277:2012 with a mix of nitric acid, hydrochloric acid and hydrogen peroxide, followed by detection and quantification using cold vapor atomic fluorescence spectroscopy |
Uranium-235 Uranium-238 | Swedish Match: FDA method: “CFSAN/ORS/DBC/CHCB April 25, 2011” (ICP-MS technique) (Modified) | The metals were released from a matrix through microwave digestion using Milli-Q water, nitric acid with a concentration of 67–69%, and hydrogen peroxide. The obtained solution was analyzed using an ICP-MS |
Polonium-210 | Labstat/Maxxam | Detected using alpha emission spectrometry |
NAB (N-Nitrosoanabasine) NAT (N-Nitrosoanabatine) NNK (4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone) NNN (N-Nitrosonornicotine) | Eurofins: In-house LW0A0 | Extracted with ethyl acetate in presence of d-labelled specific internal standards, followed by detection and quantification with high-performance liquid chromatography using a C18, 3 µm column and a tandem mass-spectrometry (HPLC–MS/MS), positive polarity |
NDMA (N-Nitrosodimethylamine) | Eurofins: In-house LP061 | Extracted with ethyl acetate in the presence of a specific internal standard, followed by detection and quantification with HPLC–MS/MS, positive polarity. An HSS T3, 1.8 µm APCI column was used |
NDELA (N-Nitrosodiethanolamine) | Extracted with Milli-Q water with the addition of an internal standard (NDELA-d8). The water extract was cleaned up using two different SPE-columns following separation and quantification using ultra performance liquid chromatography-MS/MS (UPLC-MS/MS) | |
NMOR (N-Nitrosomorpholine) NPIP (N-Nitrosopiperidine) NPYR (N-Nitrosopyrrolidine) NSAR (N-Nitrososarcosine) | Swedish Match: In-house, based on [32] | After addition of the internal standards (NSAR-d3, NMOR-d4, NPYR-d4, and NPIP-d4) the nitrosamines were extracted using 2% formic acid in acetonitrile. The extracts were diluted with 2% formic acid in water and filtered. Separation and quantification were performed using UPLC-MS/MS |
Benz[a]antracene Benzo[b,k]fluoranthene Chrysene Dibenz[a,h]anthracene Indeno[1,2,3-cd]pyrene Naphthalene | Eurofins: In-house SLF92 | Extracted with acetone in presence of specific d-labelled internal standards. The extracts were transferred to a hexane solution, followed by detection and quantification with gas chromatography-mass spectrometry, GC–MS. A J&W DB-5 ms GC column 0.18 µm was used |
B(a)P (Benzo[a]pyrene) | Eurofins: In-house LW0R7 | Extraction was performed with methanol in presence of a d-labelled specific internal standard, followed by detection and quantification with HPLC-FLD |
Nitrite | Eurofins: In-house LW 091 | Extracted in Milli-Q water derivatized with sulfanilamide and naphtylethylendiamine hydrochloride and analyzed as a red complex at 540 nm |
Acetaldehyde Crotonaldehyde Formaldehyde | Eurofins: CORESTA recommended method No. 86 [33] | Extraction and derivatization were performed in the presence of specific internal standards in a two-phase-system consisting of an aqueous buffer and isohexane using DNPH as a derivatization agent, followed by detection and quantification on UPLC-MS/MS |
Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 | Eurofins: EN 14123 (mod) | Extracted using acetonitrile/methanol/water and transferred to a phosphatic buffer saline and cleaned using a monoclonal antibody affinity column. After elution from the column the aflatoxins were post-derivatized followed by detection and quantification using high performance liquid chromatography with fluorescence detection (HPLC-FLD) |
Ochratoxin A | Eurofins: NMKL 143 | Extracted with a mix of acetonitrile and water, followed by a concentration step on a preparative column based on monoclonal antibody technology. The eluate was subsequently analyzed by liquid chromatography with fluorescence detection |
Coumarin | Eurofins: In-house method | Extracted in 50% ethanol in presence of a specific internal standard, followed by detection and quantification on UPLC-MS/MS, positive polarity. A BEH, 1.7 µm column was used |
Ethyl carbamate | Eurofins: In-house method | Extracted with Milli-Q water in presence of a specific internal standard, followed by detection and quantification by UPLC-MS/MS, positive polarity. A HSS T3, 1.8 µm column was used |
Ammonia | Eurofins: In-house LW0A3 | Extracted with Milli-Q water and mixed with salicylate and dichlor-isocyanate in presence of sodium nitroprusside to form a blue complex detectable at 660 nm |
Nicotine | Eurofins: Health Canada Official method T301 (Modified) | Extracted with alkaline methanol in presence of a specific d-labelled internal standard |
Anabasine Nornicotine | Swedish Match: CORESTA recommended method No. 62 [34] (Modified) | Mixed with sodium hydroxide and extracted using an extraction solution with methyl tert-butyl ether and quinoline as an internal standard. Separation and quantitation were performed using a gas chromatograph fitted with a capillary column and flame ionization detector |
pH | Eurofins: CORESTA recommended method No. 69 [35] | Diluted with milli-q water 1:10, stirred for 5 min and measured with pH-meter |
Unprotonated nicotine | Swedish Match | Calculated according to [36] |