Application of Box-Behnken experimental design and response surface methodology for selecting the optimum RP-HPLC conditions for the simultaneous determination of methocarbamol, indomethacin and betamethasone in their pharmaceutical dosage form

Table 7 Statistical analysis of the results obtained by the proposed method and the reference methods

Statistical term	MTL		IND		BET
Statistical term	Reference method^a	Proposed method	Reference method^a	Proposed method	Reference method^a	Proposed method
Mean%	99.90	100.41	100.43	100.86	100.44	100.85
S.D±	0.71	0.6	0.81	0.86	0.41	0.73
S.E±	0.29	0.25	0.33	0.35	0.17	0.30
R.S.D%	0.71	0.6	0.80	0.85	0.41	0.72
n	6	6	6	6	6	6
V^b	0.51	0.36	0.65	0.74	0.17	0.53
t(2.57)^c		1.33		0.70		2.29
F(5.05)^c		0.71		1.14		3.12

No significant difference between the proposed methods and reference methods was found using the Student’s t-test and variance ratio F-test. ^a Reference methods for MTL, IND, and BET using HPLC methods according to USP 43-NF38 2019. ^b Variance, ^c Values in parentheses are the critical t- and F-values at P = 0.05

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