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Table 1 The effect of addition order on the fluorescence intensity of Tb+3—8HQ and Tb+3—8HQ—PRU (2 × 10–5 M Tb+3, 6 × 10–5 M 8HQ, 300 ng / mL PRU)

From: Novel sensing probe using Terbium-sensitized luminescence and 8-hydroxyquinoline for determination of prucalopride succinate: green assessment with Complex-GAPI and analytical Eco-Scale

Addition order

Fluorescence intensity

Standard deviation (SD)

PRU, 8HQ, Tb+3, buffer

303

0.72

PRU, Tb+3, 8HQ, buffer**

463

0.35

PRU, Tb+3, buffer, 8HQ

298

0.29

PRU, 8HQ, buffer, Tb+3

336

0.51

Tb+3, 8HQ, PRU, buffer

299

0.37

Tb+3, 8HQ, buffer, PRU

324

0.39

  1. **The selected order of addition