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Table 6 Regression coefficients of polynomial equation along with p-value of ANOVA of central composite design

From: Quality by design approach for development and validation of a RP-HPLC method for simultaneous estimation of xipamide and valsartan in human plasma

Item

Retention time (VAL), min

Resolution^1

Coefficient

p-valuea

Coefficient

p-valuea

Intercept

5.84

 

6.19077

 

A—% MeOH

−1.09822

 < 0.0001 

−1.44686

 < 0.0001

B—Flow rate

−0.519041

 < 0.0001

−0.0768198

0.0015

AB

0.0925

0.0837

0.1

0.0025

A2

0.17625

0.0015

–

–

B2

0.04875

0.2040

–

–

Model

Quadratic

 < 0.0001

2FI

 < 0.0001

Adjusted R2

0.9917

0.9983

  1. abold p-values indicates significant effect
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BMC Chemistry

ISSN: 2661-801X

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