Table 3 Chromatographic conditions for the proposed HPLC method for estimation of XIP and VAL

Column

Thermo Scientific^® BDS Hypersil C₈ 5 µm (250 × 4.60 mm)

Mobile phase

Isocratic binary mobile phase of MeOH: 0.025 M KH₂PO₄ adjusted to pH 3 using ortho-phosphoric acid (64.5: 35.5, v/v), filtered and degassed using 0.45 µm membrane filter

UV detection, nm

240

Flow rate, ml/min

1.2

Injected volume, µl

10

Pressure, psig

98

Temperature

Ambient