Std. | Run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Factor 5 | Response 1 | Response 2 |
---|---|---|---|---|---|---|---|---|
A: pH | B: % MeOH | C: Flow rate (mL/min) | D: Detection wavelength (nm) | E: Buffer conc (Mm) | Resolution | Retention time (VAL) (min) | ||
5 | 1 | 4 | 58 | 1.2 | 250 | 0.05 | 7.6 | 6.91 |
9 | 2 | 4 | 58 | 1 | 250 | 0.025 | 7.56 | 8 |
7 | 3 | 4 | 63 | 1.2 | 230 | 0.025 | 4.31 | 4.56 |
4 | 4 | 3 | 63 | 1.2 | 230 | 0.05 | 5.86 | 5.2 |
8 | 5 | 4 | 58 | 1 | 230 | 0.05 | 7.75 | 8.2 |
2 | 6 | 3 | 58 | 1 | 250 | 0.05 | 9.13 | 9.27 |
10 | 7 | 3 | 63 | 1 | 230 | 0.025 | 3.9 | 5.23 |
1 | 8 | 4 | 63 | 1 | 250 | 0.05 | 4.6 | 5.5 |
6 | 9 | 3 | 58 | 1.2 | 230 | 0.025 | 8.53 | 7.68 |
3 | 10 | 3 | 63 | 1.2 | 250 | 0.025 | 3.8 | 4.36 |