Table 5 Comparison between the different reported LC–MS/MS methods for CAB in plasma [17, 18] and the new suggested one
Analyte/application | Volume of human plasma | Extraction solvent composition/volume | Blood Volume | Internal standard | Components of Mobile system | Flow rate (mL/min) | Retention time/total run time | Stationary phase | Linear range of CAB (pg/mL) | The AGREE score | References |
|---|---|---|---|---|---|---|---|---|---|---|---|
CAB alone/applied for pure CAB only in plasma samples but not for human volunteers | 150 µL | A mixture of methylene chloride/isooctane, 2:3/2.5 mL | 1 mL | Deutrated C14 labeled CAB | Aqueous solution of ammonium formate (10 mM, pH 3): acetonitrile (70/30, v/v) | 0.5 | 4.26 min/4.27 min | A µ-Bondapak C18 (3.9 × 150 mm, 10 μm) | 1.86–124.0 | 0.36 | Allievi and Dostert [21] |
CAB and L-dopa/for schizophrenic patients | 100 µL | Acetonitrile, 20 mM ammonium formate liquid (90:10, v/v)/50 µL | Not mentioned | Deutrated C14 CAB | Gradient elution mode started with 20; 80 V, V acetonitrile; water (containing 20 mM ammonium formate) for 3 min | 0.2 | 7.4 min/2.5 min | RP-COSMOSIL C8-MS column (2 mm × 150 mm, 5-mm diameter, Kyoto, Japan) | 5–250 | 0.47 | Igarashi et al. [22] |
CAB alone/in pharmaceutical product analysis and pharmacokinetics studies | 500 µL | Diethyl ether/3 mL | 5 mL | Quetiapine | Isocratic mode, methanol: 20 mm ammonium acetate (70: 30, V/V) | 0.75 | 2.6 min/5.5 min | Agilent eclipse reversed-phase C18 (3.5 µm, 4.6 × 100 mm) column | 2–200 | 0.63 | Our new method |