A validated LC–MS/MS method for analysis of Cabergoline in human plasma with its implementation in a bioequivalent study: investigation of method greenness

Table 3 Results of extraction absolute recoveries and matrix effects for the analysis of CAB and QUE by the novel LC/MS/MS method in human plasma

QC levels	Extraction absolute recoveries %		Matrix effects
QC levels	CAB	QUE (IS)	CAB	QUE (IS)
LQC	76.73 ± 11.46	58.2 ± 6.20	110.91 ± 12.43	91.46 ± 2.53
MQC(A)	60.55 ± 1.84	66.85 ± 0.39	–	–
MQC(B)	62.57 ± 6.33	65.49 ± 0.56	–	–
HQC	57.71 ± 3.33	63.27 ± 0.55	100.55 ± 4.95	108.36 ± 3.65

The extraction factor was used due to withdrawal of 3 mL form 3.5 mL of diethyl ether used in extraction
Mean percentage of absolute recoveries and RSDs were estimated for six peak areas of various plasma samples
Absolute recoveries calculations were calculated by dividing mean area of extracted samples over mean area of un-extracted samples then multiplied by the extraction factor 1.167 and 100 (Ex. recovery of analyte at low concentration = (12,371.5/18815.5) * 1.167 * 100 = 76.73%)

ISSN: 2661-801X