Table 1 Validation data for determination of MZH and PYH by the proposed methods

Wavelength (nm)

230

230 and 245

226

290

Linearity (µg/mL)

5–50

5–50

5–50

5–50

Slope

0.03

0.02

0.22

0.04

Intercept

− 0.02

− 0.01

4.12

− 0.04

Correlation coefficient (R)

0.9998

0.9997

0.9995

0.9999

LOD (µg/mL)

0.52

0.87

1.11

0.43

LOQ (µg/mL)

1.60

2.65

3.37

1.31

Accuracy (recovery% ± SD)^a

100.32 ± 0.86

99.40 ± 1.63

99.07 ± 1.11

100.46 ± 1.22

Precision (% RSD)

 Intra-day

0.97

0.54

0.81

0.77

 Inter-day

1.20

0.86

1.03

0.93