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Table 2 Parameters required for system suitability tests of TLC densitometric method

From: A green TLC densitometric method for the simultaneous detection and quantification of naphazoline HCl, pheniramine maleate along with three official impurities

Parameter NPZ impurity B NPZ PHN PHN impurity A PHN impurity B
Rf 0.18 0.35 0.49 0.63 0.83
Resolution (Rs) NA 1.50 1.58 1.40 1.64
Tailing factor (T) 1.50 0.80 1.30 1.20 1.20
Retention factor (k')a 4.56 1.86 1.04 0.59 0.20
Selectivity factor (α)b NA 2.45 1.79 1.76 2.95
Column efficiency (N)c 262.44 196.00 635.04 425.11 737.86
Height equivalent to theoretical plate (mm) 0.034 0.046 0.014 0.021 0.012
  1. aRetention factor (k') = (1 − Rf)/Rf
  2. bCalculation of α = k'2/k'1
  3. cColumn efficiency (N) = 16 (z/w)2, where z is the migration length of the spot, w is the spot width