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Table 1 The obtained resolution values during mobile phase optimization

From: A green TLC densitometric method for the simultaneous detection and quantification of naphazoline HCl, pheniramine maleate along with three official impurities

Experiment No. Mobile phase composition Rs1a Rs2a Rs3a Rs4a
1 Methanol–ethyl acetate (3.5:6.5, v/v) 1.32 1.24 1.34 0.57
2 Methanol–ethyl acetate (3.0:7.0, v/v) 1.35 1.33 1.37 0.62
3 Methanol–ethyl acetate (2.5:7.5, v/v) 1.42 1.37 1.40 0.70
4 Methanol–ethyl acetate (2.0:8.0, v/v) 1.47 1.39 1.45 0.83
5 Methanol–ethyl acetate–chloroform (2.0:8.0:1.0, v/v/v) 1.49 1.51 1.13 0.75
6 Methanol–ethyl acetate–chloroform (2.0:8.0:0.8, v/v/v) 1.52 1.53 1.22 0.78
7 Methanol–ethyl acetate–chloroform (2.0:8.0:0.5, v/v/v) 1.53 1.54 1.27 0.89
8 Methanol–ethyl acetate–chloroform (2.0:8.0:0.2, v/v/v) 1.55 1.57 1.35 0.94
9 Methanol–ethyl acetate– acetone (2.0:8.0:1.0, v/v/v) 1.46 1.44 1.21 1.13
10 Methanol–ethyl acetate– acetone (2.0:8.0:0.8, v/v/v) 1.47 1.47 1.23 1.17
11 Methanol–ethyl acetate– acetone (2.0:8.0:0.5, v/v/v) 1.49 1.48 1.27 1.22
12 Methanol–ethyl acetate– acetone (2.0:8.0:0.2, v/v/v) 1.51 1.49 1.36 1.28
  1. aRs1, Rs2, Rs3 and Rs4 are the obtained resolutions between NPZ impurity B & NPZ, NPZ & PHN, PHN & PHN impurity A and PHN impurity A & PHN impurity B, respectively