Proposed HPLC method | Reported HPLC method [11] | Reference HPLC method [15] | |||
---|---|---|---|---|---|
Reagents | Penalty points | Reagents | Penalty points | Reagents | Penalty points |
Acetonitrile | 8 | Acetonitrile | 8 | Acetonitrile | 8 |
0.015 M Phosphate buffer | 0 | Methanol | 6 | Methanol | 6 |
Internal standard | 4 | Water | 0 | Water | 0 |
0.1% orthophosphoric acid | 4 | 0.05% trifluoroacetic acid | 4 | ||
∑12 | ∑18 | ∑18 |
Instruments | Penalty points | Instruments | Penalty points | Instruments | Penalty points |
---|---|---|---|---|---|
Transport | 1 | Transport | 1 | Transport | 1 |
HPLC–UV detection | 2 | HPLC–UV detection | 2 | HPLC–UV detection | 2 |
Occupational hazard | 0 | Occupational hazard | 0 | Occupational hazard | 0 |
Heater (50 ◦C) | 2 | Waste | 3 | ||
Waste | 3 | Waste | 5 | ||
∑6 | ∑10 | ∑6 | |||
Total penalty points | 18 | Total penalty points | 28 | Total penalty points | 24 |
Score | 82 | Score | 72 | Score | 76 |