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Table 3 Assay results for the estimation of the studied drugs in their co-formulated and single eye drops

From: Green HPLC method with time programming for the determination of the co-formulated eye drops of tafluprost and timolol in their challengeable ratio

Ophthalmic formulation

Proposed method

Manufacturer and reference methods

[15, 44]

 

Amount taken

(µg mL−1)

% found

% found

Saflutan®

(contains TFL 15 µg mL−1)

1.5

98.07

101.71

 

3.0

99.58

98.13

 

6.0

98.11

100.94

X̅ ± S.D

 

98.59 ± 0.86

100.26 ± 1.88

t

 

1.40 (2.78)

 

F

 

4.78 (19.00)

 

Targotimol® (contains TIM 5 mg mL−1)

80.0

101.94

98.90

 

250.0

99.89

101.36

 

1000.0

98.03

99.51

X̅ ± S.D

 

99.95 ± 1.96

99.92 ± 1.28

t

 

0.02 (2.78)

 

F

 

2.34 (19.00)

 

Taflupro plus®

(contains TFL 15 µg mL−1 and TIM 5 mg mL−1)

TFL

TIM

TFL

TIM

TFL

TIM

 

1.5

500

98.11

98.17

98.02

100.81

 

3.0

1000

98.11

98.08

100.98

98.54

 

6.0

2000

99.16

99.43

99.81

100.92

X̅ ± S.D

  

98.46 ± 0.61

98.56 ± 0.75

99.57 ± 1.45

100.09 ± 1.34

t

  

1.22 (2.78)

1.73 (2.78)

  

F

  

5.65 (19.00)

3.19 (19.00)

  
  1. Each result is the average of three replicate estimations
  2. The theoretical t and F values (P = 0.05) are between parentheses [52]