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Table 2 Assay results for the estimation of the studied drugs in different laboratory prepared mixtures in their pharmaceutical ratios

From: Green HPLC method with time programming for the determination of the co-formulated eye drops of tafluprost and timolol in their challengeable ratio

Parameter

Proposed method

Manufacturer and reference methods

[15, 44]

 

Amount taken

(µg mL−1)

Amount found

(µg mL−1)

% found

% found

TFL

TIM

TFL

TIM

TFL

TIM

TFL

TIM

 

1.5

500.0

1.471

490.278

98.07

98.06

98.71

98.62

 

3.0

1000.0

2.987

981.444

99.58

98.19

101.11

101.65

 

6.0

2000.0

5.887

1995.944

98.11

99.79

99.80

99.41

X̅ ± S.D

    

98.59 ± 0.86

98.68 ± 0.96

99.87 ± 1.20

99.89 ± 1.57

t

    

1.50 (2.78)

1.14 (2.78)

  

F

    

1.95 (19.00)

2.67 (19.00)

  
  1. Each result is the average of three replicate estimations
  2. The theoretical t and F values (P = 0.05) are between parentheses [52]