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Table 5 Data of the Specifity Study of NCT and TRT for the Proposed Method

From: Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

Ratio (TRT: NCT) Tretinoin concentration (µg/mL) Nicotinamide (80 µg/mL) recovery%
D1 253 nm D2 245 nm D2 269 nm
1:40 2.0 100.00 100.00 100.00
1:20 4.0 99.42 100.29 100.70
1:27 3.0 99.77 100.47 99.11
1:80 1.0 99.39 100.63 100.11
1:160 0.5 100.24 100.58 99.85
Range % 99.39–100.24 100.29–100.63 99.11–100.70