Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

Table 5 Data of the Specifity Study of NCT and TRT for the Proposed Method

Ratio (TRT: NCT)	Tretinoin concentration (µg/mL)	Nicotinamide (80 µg/mL) recovery%
Ratio (TRT: NCT)	Tretinoin concentration (µg/mL)	D₁ 253 nm	D₂ 245 nm	D₂ 269 nm
1:40	2.0	100.00	100.00	100.00
1:20	4.0	99.42	100.29	100.70
1:27	3.0	99.77	100.47	99.11
1:80	1.0	99.39	100.63	100.11
1:160	0.5	100.24	100.58	99.85
Range %		99.39–100.24	100.29–100.63	99.11–100.70

ISSN: 2661-801X