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Table 3 Data of the Accuracy Study of NCT and TRT for the Proposed Method

From: Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

NCT taken concentration (µg/mL) NCT mean percentage ± RSD %a TRT taken concentration (µg/mL) TRT mean percentage ± RSD%a
Wavelength, Dev order 253 nm D1 245 nm D2 269 nm D2 348 nm D0
30 101.22 ± 0.72 99.10 ± 0.90 100.92 ± 0.68 0.75 100.49 ± 1.85
60 101.39 ± 0.63 101.48 ± 0.55 101.06 ± 0.64 1.50 100.40 ± 1.47
90 99.68 ± 0.57 101.30 ± 1.34 100.35 ± 0.07 2.25 99.78 ± 0.91
Mean percentage ± RSD%b 100.76 ± 0.98 100.63 ± 1.21 100.78 ± 0.57 Mean percentage ± RSD % 100.22 ± 1.67
Range%b 99.03–102.12 98.62–101.87 100.27–101.71 Range % 98.63–101.89
  1. aThree repetitions of each concentration were analyzed with nine total repetitions
  2. bFrom all 9 repetitions of each wavelength