Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

Table 3 Data of the Accuracy Study of NCT and TRT for the Proposed Method

NCT taken concentration (µg/mL)	NCT mean percentage ± RSD %^a			TRT taken concentration (µg/mL)	TRT mean percentage ± RSD%^a
Wavelength, Dev order	253 nm D₁	245 nm D₂	269 nm D₂	TRT taken concentration (µg/mL)	348 nm D₀
30	101.22 ± 0.72	99.10 ± 0.90	100.92 ± 0.68	0.75	100.49 ± 1.85
60	101.39 ± 0.63	101.48 ± 0.55	101.06 ± 0.64	1.50	100.40 ± 1.47
90	99.68 ± 0.57	101.30 ± 1.34	100.35 ± 0.07	2.25	99.78 ± 0.91
Mean percentage ± RSD%^b	100.76 ± 0.98	100.63 ± 1.21	100.78 ± 0.57	Mean percentage ± RSD %	100.22 ± 1.67
Range%^b	99.03–102.12	98.62–101.87	100.27–101.71	Range %	98.63–101.89

^aThree repetitions of each concentration were analyzed with nine total repetitions
^bFrom all 9 repetitions of each wavelength

ISSN: 2661-801X