From: Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations
TRT concentrations (µg/mL)
Percentage%
t0 = 0 h
t1 = 24 h
t2 = 48 h
t3 = 72 h
0.75
100.00
99.91
101.44
103.05
1.50
99.60
101.19
102.20
2.25
99.82
100.85
102.33
In the accepted range 98–102%
Yes
No