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Table 1 Analytical performance data for the proposed method

From: Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

Component parameter Nicotinamide Tretinoin
Wavelength (nm), Dev order 253, D1 245, D2 269, D2 348, D0
Linearity-range (μg/mL) 10–80 20–120 10–120 0.5–5.0
Regression equation y = 0.0068x + 0.0050 y = 0.0022x + 0.0037 y = 0.0061x—0.0028 y = 0.1556x + 0.0003
Correlation coefficient r2 0.9996 0.9998 0.9999 0.9999
LOD μg/mL 4.50 5.60 1.50 0.007
LOQ μg/mL 13.60 16.80 4.30 0.021