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Table 1 Analytical performance data for the proposed method

From: Development and validation of derivative UV spectroscopic method for simultaneous estimation of nicotinamide and tretinoin in their binary mixtures and pharmaceutical preparations

Component parameter

Nicotinamide

Tretinoin

Wavelength (nm), Dev order

253, D1

245, D2

269, D2

348, D0

Linearity-range (μg/mL)

10–80

20–120

10–120

0.5–5.0

Regression equation

y = 0.0068x + 0.0050

y = 0.0022x + 0.0037

y = 0.0061x—0.0028

y = 0.1556x + 0.0003

Correlation coefficient r2

0.9996

0.9998

0.9999

0.9999

LOD μg/mL

4.50

5.60

1.50

0.007

LOQ μg/mL

13.60

16.80

4.30

0.021