Table 3 Intra-day precision, inter-day precision and recovery studies of the developed method for the determination of antibiotics in chicken tissue
Analyte | Spiked amount μg kg−1 | Repeatabilitya (n = 3 determinations) | Reproducibilityb (n = 3 determinations) | ||||
|---|---|---|---|---|---|---|---|
Measured ± SD (μg kg−1) | % RSD | Recovery (%) | Measured ± SD (μg kg−1) | % RSD | Recovery (%) | ||
GENTA | 50 | 48.0 ± 0.7 | 3.0 | 94.4 | 50.5 ± 2.5 | 2.1 | 101 |
100 | 99.5 ± 2.1 | 1.1 | 99.0 | 99.8 ± 1.4 | 1.1 | 99.8 | |
150 | 149 ± 1.1 | 1.3 | 99.2 | 147 ± 1.2 | 1.1 | 99.0 | |
AMOX | 25 | 24.3 ± 0.9 | 3.7 | 97.2 | 24.8 ± 1.3 | 5.3 | 99.4 |
50 | 50.5 ± 1.2 | 2.4 | 101 | 49.5 ± 2.0 | 4 | 99.0 | |
75 | 75.8 ± 1.1 | 1.4 | 101 | 75.7 ± 1.8 | 2.3 | 101 | |
PEN G | 25 | 24.9 ± 1.6 | 6.3 | 99.6 | 23.9 ± 1.8 | 7.5 | 95.6 |
50 | 49.7 ± 2.1 | 4.2 | 99.4 | 50.3 ± 3.8 | 7.6 | 101 | |
75 | 75.3 ± 2.5 | 3.3 | 100 | 76.3 ± 3.0 | 3.9 | 102 | |
AMPI | 25 | 23.9 ± 2.0 | 3.3 | 96.0 | 23.5 ± 0.3 | 3.7 | 95.5 |
50 | 50.9 ± 2.4 | 4.7 | 102 | 51.1 ± 2.1 | 4.2 | 102 | |
75 | 74.7 ± 2.8 | 1.3 | 101 | 74.9 ± 1.1 | 2.4 | 100 | |
ERYTHRO | 100 | 99.4 ± 1.7 | 1.1 | 99.4 | 98.9 ± 2.3 | 1.5 | 98.9 |
200 | 200 ± 3.5 | 1.2 | 99.8 | 199 ± 3.4 | 1.1 | 99.3 | |
300 | 299 ± 3.9 | 0.9 | 99.8 | 299 ± 3.2 | 0.7 | 99.0 | |
SULFA | 50 | 47.0 ± 0.8 | 3.1 | 95.6 | 50.2 ± 3.5 | 2.4 | 101 |
100 | 99.9 ± 2.3 | 2.1 | 98.9 | 99.4 ± 2.4 | 1.5 | 99.3 | |
150 | 149 ± 1.7 | 1.2 | 99.0 | 146 ± 1.6 | 1.3 | 97.0 | |
CIPRO | 50 | 50.7 ± 0.7 | 1.4 | 101 | 49.8 ± 1.0 | 2.1 | 99.6 |
100 | 98.1 ± 3.2 | 3.5 | 98.7 | 101 ± 1.1 | 2.3 | 101 | |
150 | 144 ± 1.3 | 1.1 | 94.0 | 142 ± 1.3 | 1.4 | 95.0 | |