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Table 3 Intra-day precision, inter-day precision and recovery studies of the developed method for the determination of antibiotics in chicken tissue

From: Development and validation of liquid chromatography method for simultaneous determination of multiclass seven antibiotic residues in chicken tissues

Analyte Spiked amount μg kg−1 Repeatabilitya (n = 3 determinations) Reproducibilityb (n = 3 determinations)
Measured ± SD (μg kg−1) % RSD Recovery (%) Measured ± SD (μg kg−1) % RSD Recovery (%)
GENTA 50 48.0 ± 0.7 3.0 94.4 50.5 ± 2.5 2.1 101
100 99.5 ± 2.1 1.1 99.0 99.8 ± 1.4 1.1 99.8
150 149 ± 1.1 1.3 99.2 147 ± 1.2 1.1 99.0
AMOX 25 24.3 ± 0.9 3.7 97.2 24.8 ± 1.3 5.3 99.4
50 50.5 ± 1.2 2.4 101 49.5 ± 2.0 4 99.0
75 75.8 ± 1.1 1.4 101 75.7 ± 1.8 2.3 101
PEN G 25 24.9 ± 1.6 6.3 99.6 23.9 ± 1.8 7.5 95.6
50 49.7 ± 2.1 4.2 99.4 50.3 ± 3.8 7.6 101
75 75.3 ± 2.5 3.3 100 76.3 ± 3.0 3.9 102
AMPI 25 23.9 ± 2.0 3.3 96.0 23.5 ± 0.3 3.7 95.5
50 50.9 ± 2.4 4.7 102 51.1 ± 2.1 4.2 102
75 74.7 ± 2.8 1.3 101 74.9 ± 1.1 2.4 100
ERYTHRO 100 99.4 ± 1.7 1.1 99.4 98.9 ± 2.3 1.5 98.9
200 200 ± 3.5 1.2 99.8 199 ± 3.4 1.1 99.3
300 299 ± 3.9 0.9 99.8 299 ± 3.2 0.7 99.0
SULFA 50 47.0 ± 0.8 3.1 95.6 50.2 ± 3.5 2.4 101
100 99.9 ± 2.3 2.1 98.9 99.4 ± 2.4 1.5 99.3
150 149 ± 1.7 1.2 99.0 146 ± 1.6 1.3 97.0
CIPRO 50 50.7 ± 0.7 1.4 101 49.8 ± 1.0 2.1 99.6
100 98.1 ± 3.2 3.5 98.7 101 ± 1.1 2.3 101
150 144 ± 1.3 1.1 94.0 142 ± 1.3 1.4 95.0
  1. aStudied by spiking the tissue samples on the same day, under the same experimental conditions
  2. bEvaluated by spiking the indicated concentration levels in triplicate for six consecutive days