Fig. 4From: Synthesis of novel indole-isoxazole hybrids and evaluation of their cytotoxic activities on hepatocellular carcinoma cell linesDetection of apoptosis in HCC cells after treatment with selected compounds using IC100 concentrations for 48Â h. A Fluorescent microscopy images of HCC cells stained with Hoechst 33258 where condensed nuclei and nuclear blebbings are visible in light blue color. B Annexin-V/PI staining of Huh7 and Mahlavu cells analyzed with flow cytometry. Bar graphs indicate percentage of live and apoptotic cells in each group. C Western blot analysis of cleaved-PARP protein indicated with a yellow arrow in Huh7 and Mahlavu cells. Calnexin was used as loading controlBack to article page