Table 4 Linear range, Coefficient of corelation, MRL/MRPL, CCα, CCβ, LOD and LOQ
From: A new LC–MS/MS method for multiple residues/contaminants in bovine meat
Analytes | Linear range (μg/kg) | R2 | MRL*/MRPL** (μg/kg) | CCα (μg/kg) | CCβ (μg/kg) | LOD (μg/kg) | LOQ (μg/kg) | Internal standard |
|---|---|---|---|---|---|---|---|---|
β-agonists | ||||||||
Clenbuterol | 0.05–0.75 | 0.990 | 0.1** | 0.068 | 0.083 | 0.059 | 0.081 | Clenbuterol-d6 |
Brombuterol | 0.05–0.75 | 0.990 | 0.1** | 0.070 | 0.096 | 0.066 | 0.096 | Brombuterol-d9 |
Mabuterol | 0.05–0.75 | 0.999 | 0.1** | 0.067 | 0.091 | 0.059 | 0.100 | Mabuterol-d9 |
Clenpenterol | 0.125–0.75 | 0.995 | 0.5** | 0.298 | 0.421 | 0.290 | 0.410 | Clenpenterol-d5 |
Isoxsuprine | 0.125–0.75 | 0.996 | 0.5** | 0.313 | 0.422 | 0.281 | 0.440 | Isosuxprin-d5 hemifumarat |
Cimbuterol | 0.125–0.75 | 0.982 | 0.5** | 0.300 | 0.413 | 0.281 | 0.418 | Cimbuterl-d9 |
Ractopamine | 0.125–0.75 | 0.981 | 1.0** | 0.192 | 0.264 | 0.218 | 0.307 | Ractopamin-d6 |
Salbutamol | 0.25–2.50 | 0.983 | 5.0** | 1.083 | 1.398 | 0.546 | 0.732 | Salbutamol-d9 |
Zilpaterol | 0.25–2.50 | 0.990 | 5.0** | 0.677 | 0.945 | 0.608 | 0.894 | Zilpaterol-d7 |
Terbutalin | 0.25–2.50 | 0.982 | 10.0** | 0.840 | 1.111 | 0.616 | 0.897 | Terbutalin-d9 |
Anabolics | ||||||||
Testosteron | 1.0–50.0 | 0.992 | – | 0.325 | 0.407 | 0.264 | 0.378 | |
Boldenon | 1.0–50.0 | 0.998 | 1.0** | 0.428 | 0.522 | 0.308 | 0.419 | |
Clostebol | 1.0–50.0 | 0.983 | – | 0.742 | 1.000 | 0.597 | 0.782 | |
Methyltestosterone | 1.0–50.0 | 0.9 | 1.0** | 0.695 | 0.855 | 0.504 | 0.690 | |
Stanozolol | 1.0–50.0 | 0.981 | 1.0** | 0.548 | 0.745 | 0.498 | 0.672 | |
19 nortestosterone | 1.0–50.0 | 0.990 | 1.0** | 0.442 | 0.580 | 0.376 | 0.511 | |
Lactones | ||||||||
Zeranol | 1.0–50.0 | 0.990 | 1.0** | 0.338 | 0.473 | 0.296 | 0.423 | |
Taleranol | 1.0–50.0 | 0.999 | – | 0.715 | 0.900 | 0.578 | 0.792 | |
β-lactams | ||||||||
Amoxicillin | 25.0–200.0 | 0.991 | 50* | 55.12 | 66.40 | 27.82 | 32.62 | Penicilin G–d7 |
Oxacillin | 50.0–500.0 | 0.990 | 300* | 312.33 | 359.22 | 46.22 | 49.53 | Penicilin G–d7 |
Cloxacilin | 25.0–200.0 | 0.997 | – | 52.78 | 57.15 | 19.21 | 22.71 | Penicilin G–d7 |
Benzylpenicillin | 25.0–200.0 | 0.983 | 50* | 56.32 | 66.43 | 17.21 | 19.46 | Penicilin G–d7 |
Ampicillin | 25.0–200.0 | 0.990 | 50* | 54.12 | 59.31 | 22.31 | 24.36 | Penicilin G–d7 |
Cephalosporins | ||||||||
Ceftiofur | 300.0–3000.0 | 0.991 | 1000* | 1114.21 | 1272.8 | 291.36 | 328.13 | |
Cephalexin | 50.0–500.0 | 0.990 | 200* | 224.15 | 248.64 | 42.88 | 47.15 | |
Fluoroquinolones | ||||||||
Enrofloxacin | 25.0–200.0 | 0.990 | 100* | 105.22 | 113.94 | 24.84 | 28.07 | Flunixin d3 |
Ciprofloxacin | 25.0–200.0 | 0.992 | 100* | 108.17 | 132.72 | 25.32 | 29.91 | Flunixin d3 |
Tetracyclines | ||||||||
Oxytetracyclin | 25.0–200.0 | 0.999 | 100* | 111.45 | 116.74 | 20.78 | 23.48 | |
Sulfonamides | ||||||||
Sulfachloropyridazine | 25.0–200.0 | 0.999 | 100* | 112.15 | 128.91 | 26.90 | 31.31 | |
Sulfadiazine | 25.0–200.0 | 0.983 | 100* | 108.23 | 117.26 | 27.83 | 32.45 | |
Sulfadimethoxine | 25.0–200.0 | 0.990 | 100* | 111.14 | 129.22 | 27.11 | 30.76 | |
Sulfadimidine | 25.0–200.0 | 0.990 | 100* | 104.56 | 109.21 | 26.86 | 30.69 | |
Sulfamethoxazol | 25.0–200.0 | 0.998 | 100* | 122.18 | 134.95 | 26.69 | 29.89 | |
OP pesticides | ||||||||
Carbofuran | 1.0–50.0 | 0.991 | 10* | 12.26 | 13.81 | 1.08 | 2.12 | |
Carbaryl | 25.0–200.0 | 0.990 | 50* | 54.18 | 57.33 | 26.06 | 29.15 | |
Parathion | 25.0–200.0 | 0.981 | 50* | 59.12 | 62.78 | 21.36 | 24.35 | |
Malathion | 1.0–50.0 | 0.990 | 20* | 23.13 | 27.37 | 1.54 | 3.21 | |
Diazinon | 1.0–50.0 | 0.982 | 20* | 25.47 | 31.19 | 1.48 | 2.54 | |
Dimethoate | 25.0–200.0 | 0.990 | – | 55.17 | 63.63 | 16.25 | 19.23 | |
Atrazine | 25.0–200.0 | 0.981 | – | 54.13 | 60.01 | 18.24 | 20.46 | |
Permethrin | 25.0–200.0 | 0.999 | 50* | 56.38 | 59.06 | 20.51 | 22.18 | |
Cypermethrin | 300.0–3000.0 | 0.992 | 2000* | 2103.84 | 2482.13 | 287.26 | 315.22 | |
Deltamethrin | 25.0–100.0 | 0.994 | 30* | 32.18 | 35.08 | 10.14 | 12.78 | |
Coumaphos | 1.0–50.0 | 0.991 | 20* | 22.15 | 25.49 | 2.48 | 3.76 | |
Dichlorophos | 25.0–200.0 | 0.990 | – | 56.88 | 61.36 | 19.22 | 22.43 | |
Chlorpyrifos | 1.0–50.0 | 0.981 | 10* | 13.56 | 17.08 | 1.63 | 2.44 | |
Fenvalerate | 1.0–50.0 | 0.983 | 25* | 28.12 | 33.46 | 2.28 | 3.17 | |
Mycotoxins | ||||||||
Zearalenone | 25.0–200.0 | 0.999 | – | 54.38 | 59.26 | 20.14 | 23.71 | β-zearalenol-d4 |
Ochratoxin A | 25.0–200.0 | 0.990 | – | 57.44 | 62.18 | 19.21 | 22.14 | – |