Fig. 3

RKO cell line was exposed to different doses of compound 3i for 24 h. Expression of protein: A p53 and p21 protein. B Pro-autophagic LC3 II and p62. C Pro-apoptotic proteins: Bax, Bcl-2, cleaved caspase-3, and PARP-1. Actin was used as control. D Quantification expression protein. The tests for significance were limited to ANOVA-Dunnet post-test: ∗ p < 0.01, ∗ ∗ p < 0.001, ∗ ∗ ∗ p < 0.0001 vs. control. Full-length gels are presented in Additional file 1: Fig. S1