Fig. 2

Cytotoxic effect of compound 3i. RKO cell line were exposed to 10–70 µM of 3i for 24 and 48 h and evaluated through Trypan Blue dye exclusion assay. A cell population, and B percentages of cell viability. The tests for significance were limited to ANOVA-Dunnett post-test, *p < 0.01, **p < 0.001, ***p < 0.0001 vs. control. C Cell morphology after exposure at 30, 40, and 50 µM; Horizontal bar in bottom figure = 50 µm