Table 5 Effects of different instruments, columns, column temperatures, flow rates, volume and pH on RRTs (n = 3)

RSD%

 Chromatographic Columns

38.95

36.77

35.97

-

31.67

 HPLC Instruments

1.09

1.24

3.38

-

2.34

 Column Temperatures

1.58

1.36

0.54

-

0.93

 Flow Volume of Mobile Phase

2.10

2.11

0.93

-

1.22

 Flow Rate of Mobile Phase

1.22

0.75

0.40

-

0.005

 pH Value of Mobile Phase

4.87

3.21

3.99

-

3.71

 \(\bar \chi \)

0.31

0.49

0.63

-

2.11

 S

0.001

0.001

0.001

-

0.009

 \(\bar \chi \pm 3{\rm{S}}\)

0.31 ± 0.003

0.49 ± 0.004

0.63 ± 0.002

-

2.11 ± 0.028