Table 1 1H chemical shift assignments for hyaluronan
Proton number (see Fig. 1) | Experimental High-molecular weight polymer (ppm) | Literaturea Low-molecular weight polymer (ppm) | Literatureb HA6 (ppm) |
|---|---|---|---|
N-acetyl glucosamine | |||
H1 | 4.55 | 4.55 | 4.55 |
H2 | 3.83 | 3.83 | 3.84 |
H3 | c(3.71) | c3.73 | 3.71 |
H4 | 3.51 | 3.51 | 3.52 |
H5 | 3.48 | 3.47 | 3.48 |
H6 | 3.9 | 3.91 | 3.91 |
H6ʹ | c(3.71) | 3.71 | 3.76 |
H-CH3 | 2.02 | 2.01 | 2.02 |
d-glucuronic acid | |||
H1 | 4.45 | 4.46 | 4.46 |
H2 | 3.33 | 3.34 | 3.34 |
H3 | 3.58 | 3.58 | 3.58 |
H4 | c(3.71) | 3.74 | 3.74 |
H5 | c(3.71) | 3.72 | 3.70 |