A bioanalytical UHPLC based method used for the quantification of Thymoquinone-loaded-PLGA-nanoparticles in the treatment of epilepsy

Table 2 Validation: precision and accuracy data for thymoquinone in different biomatrixes

Biomatrix	Quality controls samples	Theoretical concentration (ng mL⁻¹) or (ng g⁻¹)	Intra-batch precision			Inter-batch precision			Recovery^c (%)
Biomatrix	Quality controls samples	Theoretical concentration (ng mL⁻¹) or (ng g⁻¹)	Observed concentration (ng mL⁻¹) or (ng g⁻¹) ± S.D.	Accuracy^a (%)	Precision^b (%C.V.)	Observed concentration (ng mL⁻¹) or (ng g⁻¹) ± S.D.	Accuracy^a (%)	Precision^b (%C.V.)	Recovery^c (%)
Brain Homogenate	LOQQC	101.00	97.79 ± 1.38	96.82	1.41	96.63 ± 1.27	95.67	1.31	85.34
	LQC	290.00	276.94 ± 3.64	95.50	1.31	274.56 ± 3.11	94.68	1.13	86.47
	MQC	1000.00	979.64 ± 11.46	97.96	1.17	974.43 ± 11.17	97.44	1.15	87.61
	HQC	2000.00	1985.22 ± 27.11	99.26	1.37	1982.42 ± 26.64	99.12	1.34	88.23
Lungs Homogenate	LOQQC	101.00	97.68 ± 0.934	96.71	0.96	98.28 ± 1.14	97.31	1.16	83.14
	LQC	290.00	282.46 ± 3.75	97.40	1.33	279.99 ± 3.64	96.55	1.30	88.18
	MQC	1000.00	976.49 ± 15.47	97.65	1.58	974.94 ± 14.99	97.49	1.54	87.23
	HQC	2000.00	1981.44 ± 25.14	99.07	1.27	1980.47 ± 24.34	99.02	1.23	87.43
Plasma	LOQQC	101.00	99.64 ± 1.16	98.65	1.16	99.31 ± 1.01	98.33	1.02	88.32
	LQC	290.00	281.45 ± 3.93	97.05	1.40	279.64 ± 3.81	96.43	1.36	86.93
	MQC	1000.00	976.42 ± 18.69	97.64	1.91	972.25 ± 18.14	97.23	1.86	87.39
	HQC	2000.00	1976.48 ± 26.18	98.82	1.32	1974.14 ± 25.22	98.71	1.28	87.24

ISSN: 2661-801X