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Table 4 The four factors and three-level orthogonal experiment and ANOVA results for the determination of eleutheroside E in decoctions of the formulas of AV and ES

From: Development and application of a rapid HPLC method for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in Apocynum venetum L. and Eleutherococcus senticosus

 

Sum of squares

df

Mean square

F

P (Sig.)

Calibration model

96.34

6

16.06

21.73

0.05

Intercept

11733.95

1

11733.95

15880.77

0

Water addition

22.35

2

11.18

15.13

0.06

Decoction duration

26.50

2

13.25

17.93

0.05

Frequency of decoction

47.49

2

23.75

32.14

0.03

Error (Soaking time)

1.48

2

0.74