Skip to main content

Table 1 Results of Simplex-Centroid experimental design for total phenols and flavonoids, EC50, HEp-2 cell line and mononuclear cells

From: Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities

Solvents

Response variables

Extract

H2O

EtOH

MeOH

TPC

mgGAc/g

TFC

mgQ/g

EC50

μg/mL

HEp-2

(%)

MVC

(%)

1

0.0

50.0

50.0

339.52 ± 0.01

130.22 ± 0.02

3.85 ± 0.01

104.46 ± 0.08

214.92 ± 3.88

2

0.0

100.0

0.0

344.12 ± 0.01

128.46 ± 0.01

3.39 ± 0.01

189.90 ± 0.33

196.41 ± 18.27

3

16.7

16.7

66.6

322.78 ± 0.01

119.69 ± 0.01

4.97 ± 0.01

151.30 ± 0.04

176.45 ± 1.45

4

100.0

0.0

0.0

285.66 ± 0.01

101.86 ± 0.01

4.41 ± 0.00

74.70 ± 0.10

151.02 ± 10.00

5

0.0

100.0

0.0

412.59 ± 0.01

132.63 ± 0.03

4.13 ± 0.00

54.60 ± 0.17

232.33 ± 17.96

6

0.0

0.0

100.0

330.33 ± 0.01

137.43 ± 0.02

7.71 ± 0.00

43.37 ± 0.19

213.67 ± 11.50

7

50.0

50.0

0.0

318.51 ± 0.02

107.39 ± 0.01

8.38 ± 0.00

54.33 ± 0.30

202.03 ± 4.24

8

100.0

0.0

0.0

268.92 ± 0.00

122.76 ± 0.01

5.34 ± 0.00

63.24 ± 0.15

163.96 ± 4.79

9

16.7

66.7

16.7

312.27 ± 0.01

118.16 ± 0.02

7.58 ± 0.01

65.74 ± 0.39

148.28 ± 5.69

10

0.0

50.0

50.0

335.75 ± 0.01

131.32 ± 0.04

7.43 ± 0.01

98.20 ± 0.10

228.28 ± 3.56

11

66.6

16.7

16.7

322.12 ± 0.02

103.22 ± 0.00

5.71 ± 0.01

80.51 ± 0.04

169.66 ± 0.68

12

33.3

33.3

33.3

299.95 ± 0.03

118.68 ± 0.03

5.47 ± 0.01

71.51 ± 0.29

204.27 ± 3.09

13

50.0

0.0

50.0

164.15 ± 0.02

122.76 ± 0.00

5.00 ± 0.00

94.47 ± 0.14

290.06 ± 13.60

14

0.0

0.0

100.0

324.25 ± 0.02

127.59 ± 0.02

4.25 ± 0.00

121.87 ± 0.14

236.67 ± 20.37

  1. GAc Gallic Acid, Q quercetin