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Table 1 Results of Simplex-Centroid experimental design for total phenols and flavonoids, EC50, HEp-2 cell line and mononuclear cells

From: Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities

SolventsResponse variables
ExtractH2OEtOHMeOHTPC
mgGAc/g
TFC
mgQ/g
EC50
μg/mL
HEp-2
(%)
MVC
(%)
10.050.050.0339.52 ± 0.01130.22 ± 0.023.85 ± 0.01104.46 ± 0.08214.92 ± 3.88
20.0100.00.0344.12 ± 0.01128.46 ± 0.013.39 ± 0.01189.90 ± 0.33196.41 ± 18.27
316.716.766.6322.78 ± 0.01119.69 ± 0.014.97 ± 0.01151.30 ± 0.04176.45 ± 1.45
4100.00.00.0285.66 ± 0.01101.86 ± 0.014.41 ± 0.0074.70 ± 0.10151.02 ± 10.00
50.0100.00.0412.59 ± 0.01132.63 ± 0.034.13 ± 0.0054.60 ± 0.17232.33 ± 17.96
60.00.0100.0330.33 ± 0.01137.43 ± 0.027.71 ± 0.0043.37 ± 0.19213.67 ± 11.50
750.050.00.0318.51 ± 0.02107.39 ± 0.018.38 ± 0.0054.33 ± 0.30202.03 ± 4.24
8100.00.00.0268.92 ± 0.00122.76 ± 0.015.34 ± 0.0063.24 ± 0.15163.96 ± 4.79
916.766.716.7312.27 ± 0.01118.16 ± 0.027.58 ± 0.0165.74 ± 0.39148.28 ± 5.69
100.050.050.0335.75 ± 0.01131.32 ± 0.047.43 ± 0.0198.20 ± 0.10228.28 ± 3.56
1166.616.716.7322.12 ± 0.02103.22 ± 0.005.71 ± 0.0180.51 ± 0.04169.66 ± 0.68
1233.333.333.3299.95 ± 0.03118.68 ± 0.035.47 ± 0.0171.51 ± 0.29204.27 ± 3.09
1350.00.050.0164.15 ± 0.02122.76 ± 0.005.00 ± 0.0094.47 ± 0.14290.06 ± 13.60
140.00.0100.0324.25 ± 0.02127.59 ± 0.024.25 ± 0.00121.87 ± 0.14236.67 ± 20.37
  1. GAc Gallic Acid, Q quercetin