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Table 7 Reproducibility study at MRL, 0.5 MRL or concentration < 0.5 MRL

From: Primary validation of Charm II tests for the detection of antimicrobial residues in a range of aquaculture fish

Family

Compound

Spiking concentration (µg/kg)

Mean cpm

SDr

RSDr (%)

Tetracyclines

Chlortetracycline

25 µg/kg (0.25 MRL)

1224.5

139.2

11.4

Oxytetracycline

100 µg/kg (MRL)

1277

92.6

7.2

Macrolides

Erythromycin A

100 µg/kg (0.5 MRL)

1748.5

156.1

8.9

200 µg/kg (MRL)

1456.5

83.9

5.8

β-Lactams

Penicillin G

25 µg/kg (0.5 MRL)

1204.9

135.0

11.2

50 µg/kg (MRL)

702.1

73.0

10.4

Aminoglycosides

Streptomycin

250 µg/kg (0.5 MRL)

1110.6

98.7

8.9

500 µg/kg (MRL)

1132.6

171.4

15.1

Sulfonamides

Sulfathiazole

25 µg/kg (0.25 MRL)

943.7

78.1

8.3

100 µg/kg (MRL)

647.1

18.1

2.8

  1. SDr standard deviation under reproducibility conditions, RSDr relative standard deviation under reproducibility conditions