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Table 1 Identification of chemical constituents in water (121 °C, 15 psi), water (room temperature) and methanol extract of P. tenellus leaves

From: Pressurized hot water extraction of hydrosable tannins from Phyllanthus tenellus Roxb.

NoCompound identitym/zCompound classificationAverage relative quantification (%)
PHWEWater (room temperature)Methanol extract
1Quinic acid191Phenolic acid0.147 ± 0.050b0.197 ± 0.030b0.068 ± 0.009a
2Caffeoylmalic acid295Phenolic acid0.029 ± 0.008a0.017 ± 0.015a0.361 ± 0.050b
3Caffeic acid 3-glucoside341Phenolic acid0.055 ± 0.040b0.181 ± 0.001c0.007 ± 0.004a
41-O-(2-acetpxybenzoyl)-β-d-glucopyranuronic acid355Phenolic acid0.039 ± 0.013a0.060 ± 0.055a0.030 ± 0.001a
5o-coumaroylquinic acid337Phenolic acid0.015 ± 0.002c0.007 ± 0.001bNDa
6Coumaric acid163Phenolic acid0.025 ± 0.002bNDaNDa
7β-glucogallin331Phenolic acid0.149 ± 0.046cNDa0.019 ± 0.007b
8Caffeic acid179Phenolic acid0.024 ± 0.011cNDa0.004 ± 0.004b
92-O-caffeoyl glucarate371Phenolic acid0.063 ± 0.012bNDaNDa
102,4-Dihydroxy-6-(3-methylbutoxy)-3-(3-methylbutyl) benzaldehyde293Phenolic acid0.007 ± 0.001bNDaNDa
11Chlorogenic acid derivatives467Phenolic acid0.047 ± 0.015bNDaNDa
12Coumaroylquinic acid337Phenolic acidNDa0.017 ± 0.006b0.024 ± 0.001b
13Chlorogenic acid353Phenolic acidNDa0.026 ± 0.074b0.203 ± 0.057c
142,5-dihyroxycinnamic acid179Phenolic acidNDa0.007 ± 0.002b0.005 ± 0.001b
151-O-(3,4,5-Trihydroxybenzoyl)hexopyranose331Phenolic acidNDa0.005 ± 0.009b0.229 ± 0.017c
164-O-beta-d-glucosyl-4-coumaric acid325Phenolic acidNDa0.007 ± 0.007bNDa
17Rutin609Flavonoid0.039 ± 0.034bNDa0.020 ± 0.014b
18Quercetin glucoside463Flavonoid0.017 ± 0.004bNDa0.254 ± 0.004c
19Myricetin-rhamnoside463Flavonoid0.150 ± 0.005bNDaNDa
20Quercetin glucoside derivative649FlavonoidNDa0.006 ± 0.010b0.110 ± 0.008c
21Cyanidin malonylglucoside535FlavonoidNDaNDa0.013 ± 0.010b
22Cyanidin glucoside447FlavonoidNDaNDa0.005 ± 0.001b
23Kaempferol hexoside447FlavonoidNDaNDa0.061 ± 0.004b
246-Metoxykaempferol-3-O-hexose-O-pentose577Flavonoid0.046 ± 0.055bNDaNDa
25Apigenin pentose401FlavonoidNDaNDa0.011 ± 0.009b
26Apigenin269FlavonoidNDaNDa0.002 ± 0.000b
27Naringin579FlavonoidNDaNDa0.039 ± 0.006b
28Catechin289Flavonoid0.004 ± 0.000bNDa0.018 ± 0.003c
29Catechin derivatives279FlavonoidNDa0.003 ± 0.0050.013 ± 0.004
30Pelargonidin glucoside431AnthocyaninNDaNDa0.002 ± 0.001b
31Gallic acid169Catabolites/metabolites of hydrosable tanninNDa0.016 ± 0.016b0.030 ± 0.003b
32Gallic acid derivative521Catabolites/metabolites of hydrosable tannin0.034 ± 0.007bNDaNDa
33Gallic acid derivative537/Catabolites/metabolites of hydrosable tanninNDa0.040 ± 0.011b0.761 ± 0.045c
34Ellagic acid301Catabolites/metabolites of hydrosable tanninNDa0.008 ± 0.014b0.124 ± 0.086c
35Ellagic acid glucoside463Catabolites/metabolites of hydrosable tannin0.021 ± 0.009bNDa0.035 ± 0.032b
36Ellagic acid methyl pentoside447Catabolites/metabolites of hydrosable tanninNDaNDa0.025 ± 0.001b
37Methyl ellagic acid hexose477Catabolites/metabolites of hydrosable tanninNDaNDa0.185 ± 0.156b
38Brevifolin247Catabolites/metabolites of hydrosable tannin0.013 ± 0.003bNDa0.010 ± 0.006b
39Brevifolin carboxylic acid291Catabolites/metabolites of hydrosable tannin0.125 ± 0.021cNDa0.011 ± 0.001b
40Brevifolin conjugate Catabolites/metabolites of hydrosable tannin0.384 ± 0.029bNDaNDa
41m/z 226226Catabolites/metabolites of hydrosable tannin2.117 ± 3.667bNDaNDa
42m/z 227227Catabolites/metabolites of hydrosable tannin4.679 ± 0.546bNDaNDa
43Geraniin951Hydrosable tanninNDaNDa2.380 ± 0.035b
44Ellagitannin969Hydrosable tannin0.054 ± 0.013bNDaNDa
45Dehydrohexahydroxy diphenic acid353Hydrosable tannin0.067 ± 0.027bNDaNDa
46HHDP-galloyl-glucose633Hydrosable tannin0.407 ± 0.407bNDaNDa
47HHDP glucose783Hydrosable tanninNDaNDa0.008 ± 0.002b
48Corilagin633Hydrosable tanninNDaNDa0.555 ± 0.042b
49Strictinin633Hydrosable tanninNDaNDa0.305 ± 0.147b
50Castalin631Hydrosable tanninNDaNDa0.186 ± 0.261b
51Castalagin933Hydrosable tanninNDaNDa0.458 ± 0.050b
52Proanthocyanidin C1866Condensed tanninNDaNDa0.005 ± 0.000b
53Proanthocyanidin trimer885Condensed tanninNDaNDa0.002 ± 0.002b
  1. Triplicates of results were analyzed by one-way ANOVA with Tukey post hoc and presented as SEM. Different letters indicate significant differences between extract (p < 0.05)
  2. ND None detected