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Table 6 Application of Method 2 for the determination of the studied drugs in their pure form

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Studied drug

Proposed method

Comparison method [11]

Amount taken (ng/mL)

Amount found (ng/mL)

% Found

% Found

Column (A)

Column (B)

Column (A)

Column (B)

Column (A)

Column (B)

SFV

120.0

120.0

121.208

120.765

101.01

100.64

100.61

200.0

200.0

200.205

199.979

100.10

99.99

99.71

280.0

280.0

279.372

279.766

99.78

99.92

99.82

400.0

400.0

399.597

400.431

99.90

100.11

99.89

600.0

600.0

601.597

600.989

100.27

100.16

100.06

Mean

    

100.21

100.16

100.02

± SD

    

0.48

0.28

0.354

t-test

    

0.72

0.72

(2.31)*

F-test

    

1.86

1.57

 (6.39)*

VPS

30.0

30.0

29.134

30.045

97.11

100.15

96.41

50.0

50.0

51.597

49.907

103.19

99.81

98.24

70.0

70.0

69.821

70.056

99.74

100.08

98.10

100.0

100.0

100.119

99.523

100.12

99.52

99.58

150.0

150.0

150.321

149.981

100.21

99.99

99.47

Mean

    

100.07

99.91

98.36

± SD

    

2.16

0.25

1.29

t-test

    

2.06

0.95

(2.78)*

F-test

    

2.82 (6.39)*

0.0009 (0.156)*

 
  1. Each result is the average of three separate determinations
  2. * Figures between parentheses are the tabulated t and F values at P = 0.05 [17]