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Table 6 Application of Method 2 for the determination of the studied drugs in their pure form

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Studied drug Proposed method Comparison method [11]
Amount taken (ng/mL) Amount found (ng/mL) % Found % Found
Column (A) Column (B) Column (A) Column (B) Column (A) Column (B)
SFV 120.0 120.0 121.208 120.765 101.01 100.64 100.61
200.0 200.0 200.205 199.979 100.10 99.99 99.71
280.0 280.0 279.372 279.766 99.78 99.92 99.82
400.0 400.0 399.597 400.431 99.90 100.11 99.89
600.0 600.0 601.597 600.989 100.27 100.16 100.06
Mean      100.21 100.16 100.02
± SD      0.48 0.28 0.354
t-test      0.72 0.72 (2.31)*
F-test      1.86 1.57  (6.39)*
VPS 30.0 30.0 29.134 30.045 97.11 100.15 96.41
50.0 50.0 51.597 49.907 103.19 99.81 98.24
70.0 70.0 69.821 70.056 99.74 100.08 98.10
100.0 100.0 100.119 99.523 100.12 99.52 99.58
150.0 150.0 150.321 149.981 100.21 99.99 99.47
Mean      100.07 99.91 98.36
± SD      2.16 0.25 1.29
t-test      2.06 0.95 (2.78)*
F-test      2.82 (6.39)* 0.0009 (0.156)*  
  1. Each result is the average of three separate determinations
  2. * Figures between parentheses are the tabulated t and F values at P = 0.05 [17]