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Table 11 Assay results for the determination of VPS and SFV in their laboratory prepared mixture at their pharmaceutical ratio by Method 2

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Studied drug

Proposed method

Comparison method [11]

Amount taken (ng/mL)

Amount found (ng/mL)

% Found

% Found

Column (A)

Column (B)

Column (A)

Column (B)

Column (A)

Column (B)

SFV

120

120

120.468

119.472

100.39

99.56

99.98

200

200

200.140

195.560

100.07

97.78

98.93

280

280

279.720

273.000

99.90

97.50

100.83

400

400

399.520

393.600

99.88

98.40

 

600

600

588.120

600.420

98.02

100.07

 

Mean

    

99.65

98.66

100.85

 ±SD

    

0.93

1.12

0.95

t-test

    

0.38

1.61

(2.45)*

F-test

    

1.04

1.38

(6.94)*

VPS

30

30

30.036

29.817

100.12

99.39

101.45

50

50

49.420

50.305

98.84

100.61

100.47

70

70

68.768

70.455

98.24

100.65

99.12

100

100

99.140

100.130

99.14

100.13

 

150

150

149.910

149.100

99.94

99.40

 

Mean

    

99.26

100.04

100.35

 ±SD

    

0.78

0.62

1.17

t-test

    

1.61

0.50

(2.45)*

F-test

    

2.25

3.56

(6.94)*

  1. Each result is the average of three separate determinations
  2. * The figures between parentheses are the tabulated t and F values at P = 0.05 [17]