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Table 11 Assay results for the determination of VPS and SFV in their laboratory prepared mixture at their pharmaceutical ratio by Method 2

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Studied drug Proposed method Comparison method [11]
Amount taken (ng/mL) Amount found (ng/mL) % Found % Found
Column (A) Column (B) Column (A) Column (B) Column (A) Column (B)
SFV 120 120 120.468 119.472 100.39 99.56 99.98
200 200 200.140 195.560 100.07 97.78 98.93
280 280 279.720 273.000 99.90 97.50 100.83
400 400 399.520 393.600 99.88 98.40  
600 600 588.120 600.420 98.02 100.07  
Mean      99.65 98.66 100.85
 ±SD      0.93 1.12 0.95
t-test      0.38 1.61 (2.45)*
F-test      1.04 1.38 (6.94)*
VPS 30 30 30.036 29.817 100.12 99.39 101.45
50 50 49.420 50.305 98.84 100.61 100.47
70 70 68.768 70.455 98.24 100.65 99.12
100 100 99.140 100.130 99.14 100.13  
150 150 149.910 149.100 99.94 99.40  
Mean      99.26 100.04 100.35
 ±SD      0.78 0.62 1.17
t-test      1.61 0.50 (2.45)*
F-test      2.25 3.56 (6.94)*
  1. Each result is the average of three separate determinations
  2. * The figures between parentheses are the tabulated t and F values at P = 0.05 [17]
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