Table 10 Assay results for the determination of VPS in laboratory prepared mixture with SFV at their pharmaceutical ratio by Method 1
Combination | Proposed method | Comparison method [11] | |||||
|---|---|---|---|---|---|---|---|
Amount taken (ng/mL) | Amount found (ng/mL) | % Found | % Found | ||||
Column (A) | Column (B) | Column (A) | Column (B) | Column (A) | Column (B) | VPS | |
SFV/VPS mixture 4:1 (w/w) | 1.0 | 2.5 | 0.989 | 2.495 | 98.93 | 99.78 | 101.45 |
2.0 | 3.5 | 1.899 | 3.412 | 94.99 | 97.50 | 100.47 | |
3.0 | 5.0 | 2.947 | 4.913 | 98.22 | 98.26 | 99.12 | |
4.0 | 7.5 | 3.933 | 7.356 | 98.34 | 98.09 | ||
5.0 | 10.0 | 5.003 | 9.996 | 100.07 | 99.96 | ||
Mean | 98.11 | 98.72 | 100.35 | ||||
± SD | 1.89 | 1.09 | 1.17 | ||||
t-test | − 1.82 | 1.99 | (2.45)* | ||||
F-test | 2.62 (19.24)* | 1.15 (6.94)* | |||||