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Table 10 Assay results for the determination of VPS in laboratory prepared mixture with SFV at their pharmaceutical ratio by Method 1

From: Two novel UPLC methods utilizing two different analytical columns and different detection approaches for the simultaneous analysis of velpatasvir and sofosbuvir: application to their co-formulated tablet

Combination Proposed method Comparison method [11]
Amount taken (ng/mL) Amount found (ng/mL) % Found % Found
Column (A) Column (B) Column (A) Column (B) Column (A) Column (B) VPS
SFV/VPS mixture 4:1 (w/w) 1.0 2.5 0.989 2.495 98.93 99.78 101.45
2.0 3.5 1.899 3.412 94.99 97.50 100.47
3.0 5.0 2.947 4.913 98.22 98.26 99.12
4.0 7.5 3.933 7.356 98.34 98.09  
5.0 10.0 5.003 9.996 100.07 99.96  
Mean      98.11 98.72 100.35
± SD      1.89 1.09 1.17
t-test      − 1.82 1.99 (2.45)*
F-test      2.62 (19.24)* 1.15 (6.94)*  
  1. Each result is the average of three separate determinations
  2. * The figures between parentheses are the tabulated t and F values at P = 0.05 [17]