Parameter | No. of theoretical plates (N) | Capacity factor (k′) | Tailing factor (Tf) | |||
---|---|---|---|---|---|---|
Column (A) | Column (B) | Column (A) | Column (B) | Column (A) | Column (B) | |
Column temperature °C | ||||||
Room temperature | 2900.3 | 406.2 | 1.839 | 2.675 | 0.532 | 0.831 |
30 °C | 2941.6 | – | 1.987 | – | 0.660 | – |
40 °C | 4547.4 | 1009.7 | 2.183 | 2.038 | 0.536 | 0.900 |
50 °C | 5030.4 | 644.4 | 2.348 | 3.179 | 0.517 | 0.877 |
60 °C | – | 799.3 | – | 3.454 | – | 0.908 |
pH of mobile phase | ||||||
2.5 | 2900.3 | 406.2 | 1.839 | 2.675 | 0.532 | 0.831 |
3.3 | 3921.4 | 408.2 | 2.744 | 4.150 | 0.528 | 0.947 |
4.0 | 3305.7 | 410.6 | 3.976 | 6.425 | 0.491 | 0.924 |
Type of organic modifier of Conc 40% (v/v) | ||||||
Acetonitrile | 2720.4 | 378.8 | 1.831 | 2.583 | 0.556 | 0.867 |
Methanol | 1140.1 | 365.4 | 1.843 | 2.621 | 0.514 | 0.907 |
Ethanol | 2304.3 | 352.9 | 1.870 | 2.592 | 0.413 | 0.871 |
Ratio organic modifier: mobile phase (acetonitrile) (v/v) | ||||||
40:60 | 3145.5 | 372.7 | 1.824 | 2.554 | 0.525 | 0.825 |
60:40 | 2720.4 | 378.8 | 1.831 | 2.583 | 0.556 | 0.867 |
80:20 | 2386.9 | 314.5 | 1.844 | 2.642 | 0.552 | 0.906 |
90:10 | 2210.2 | 316.7 | 1.848 | 2.654 | 0.575 | 0.866 |
Effect of flow rate (mL/min) | ||||||
0.3 | – | 452.8 | – | 4.613 | – | 0.768 |
0.5 | 205.0 | 1.433 | 0.750 | |||
1.0 | 3551.6 | 2.376 | 0.571 | |||
1.2 | 2900.3 | – | 1.839 | – | 0.532 | – |