|
Parameter
|
No. of theoretical plates (N)
|
Capacity factor (k′)
|
Tailing factor (Tf)
|
|---|
|
Column (A)
|
Column (B)
|
Column (A)
|
Column (B)
|
Column (A)
|
Column (B)
|
|---|
|
Column temperature °C
|
|
Room temperature
|
2900.3
|
406.2
|
1.839
|
2.675
|
0.532
|
0.831
|
|
30 °C
|
2941.6
|
–
|
1.987
|
–
|
0.660
|
–
|
|
40 °C
|
4547.4
|
1009.7
|
2.183
|
2.038
|
0.536
|
0.900
|
|
50 °C
|
5030.4
|
644.4
|
2.348
|
3.179
|
0.517
|
0.877
|
|
60 °C
|
–
|
799.3
|
–
|
3.454
|
–
|
0.908
|
|
pH of mobile phase
|
|
2.5
|
2900.3
|
406.2
|
1.839
|
2.675
|
0.532
|
0.831
|
|
3.3
|
3921.4
|
408.2
|
2.744
|
4.150
|
0.528
|
0.947
|
|
4.0
|
3305.7
|
410.6
|
3.976
|
6.425
|
0.491
|
0.924
|
|
Type of organic modifier of Conc 40% (v/v)
|
|
Acetonitrile
|
2720.4
|
378.8
|
1.831
|
2.583
|
0.556
|
0.867
|
|
Methanol
|
1140.1
|
365.4
|
1.843
|
2.621
|
0.514
|
0.907
|
|
Ethanol
|
2304.3
|
352.9
|
1.870
|
2.592
|
0.413
|
0.871
|
|
Ratio organic modifier: mobile phase (acetonitrile) (v/v)
|
|
40:60
|
3145.5
|
372.7
|
1.824
|
2.554
|
0.525
|
0.825
|
|
60:40
|
2720.4
|
378.8
|
1.831
|
2.583
|
0.556
|
0.867
|
|
80:20
|
2386.9
|
314.5
|
1.844
|
2.642
|
0.552
|
0.906
|
|
90:10
|
2210.2
|
316.7
|
1.848
|
2.654
|
0.575
|
0.866
|
|
Effect of flow rate (mL/min)
|
|
0.3
|
–
|
452.8
|
–
|
4.613
|
–
|
0.768
|
|
0.5
| |
205.0
| |
1.433
| |
0.750
|
|
1.0
|
3551.6
| |
2.376
| |
0.571
| |
|
1.2
|
2900.3
|
–
|
1.839
|
–
|
0.532
|
–
|
-
\( {\text{Number of theoretical plates }}\left( {\text{N}} \right)\, = \,5.54\left( {\frac{{{t}_{{R}} }}{{{Wh}/2}} } \right)^{2} \)
- \( {\text{Tailing factor }}\left( {\text{T}} \right)\, = \,\frac{{{W}_{0.5} }}{{2{f}}} {\text{k}^{\prime}} \, = \,{{({\text{tR}} {-}{\text{ t}}0)} \mathord{\left/ {\vphantom {{({\text{tR}} {-}{\text{ t}}0)} {{\text{t}}0}}} \right. \kern-0pt} {{\text{t}}0}} \),
