Three smart spectrophotometric methods for resolution of severely overlapped binary mixture of Ibuprofen and Paracetamol in pharmaceutical dosage form

Table 3 Determination of PAR and IBU in pharmaceutical preparation and application of standard addition technique

(A) Market preparation: Cetafen^® tablet claimed to contain 325 mg PAR and 200 mg IBU
Proposed method recovery %^a						Standard addition technique
Proposed method recovery %^a						Ratio difference
Ratio difference		Mean centering		Constant center		Taken (μg/mL)		Recovery %^a
PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU
100.12 ± 0.62	101.34 ± 1.51	99.58 ± 0.53	100.52 ± 1.26	99.68 ± 0.95	100.44 ± 1.17	3.0	20.0	100.76	99.16
						3.0	20.0	101.53	100.34
						3.0	20.0	100.42	101.95

Standard addition technique
Mean centering				Constant center
Taken (μg/mL)		Recovery %^a		Taken (μg/mL)		Recovery %^a
PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU
3.0	20.0	98.14	100.96	3.0	20.0	100.37	99.02
3.0	20.0	100.33	100.55	3.0	20.0	98.43	100.55
3.0	20.0	101.08	101.13	3.0	20.0	100.85	99.89

(B) Market preparation: Parofen^® tablet claimed to contain 500 mg PAR and 400 mg IBU
Proposed method recovery %^a						Standard addition technique
Proposed method recovery %^a						Ratio difference
Ratio difference		Mean centering		Constant center		Taken (μg/mL)		Recovery %^a
PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU
98.72 ± 0.89	100.74 ± 0.56	100.58 ± 0.93	101.22 ± 1.18	9936 ± 0.52	100.36 ± 1.03	3.0	20.0	100.54	99.96
						3.0	20.0	101.24	101.31
						3.0	20.0	101.43	100.63

Standard addition technique
Mean centering				Constant center
Taken (μg/mL)		Recovery %^a		Taken (μg/mL)		Recovery %^a
PAR	IBU	PAR	IBU	PAR	IBU	PAR	IBU
3.0	20.0	100.73	100.13	3.0	20.0	101.33	99.45
3.0	20.0	101.12	99.58	3.0	20.0	100.95	99.82
3.0	20.0	100.37	100.22	3.0	20.0	100.04	98.73

ISSN: 2661-801X