Table 2 Chromatographic conditions for M. pumila flowers samples analysis
From: Dynamic changes of secondary metabolites and tyrosinase activity of Malus pumila flowers
Chromatographic conditions | Parameter |
|---|---|
Column | Inertsil ODS-SP column (4.6 mm × 250 mm, 5 μm) |
Mobile phase | Methanol (A)-0.1% phosphoric acid aqueous solution (B) 0–10 min, 5–50%A, 95–50%B 10–30 min, 50–50%A, 50–50%B 30–40 min, 50–100%A, 50–0%B 40–55 min, 100–100%A, 0–0% |
Flow rate | 0.8 mL/min |
Column temperature | 30 °C |
Detection wavelength | 270 nm |
Sample volume | 10 μL |