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Table 2 Chromatographic conditions for M. pumila flowers samples analysis

From: Dynamic changes of secondary metabolites and tyrosinase activity of Malus pumila flowers

Chromatographic conditions Parameter
Column Inertsil ODS-SP column (4.6 mm × 250 mm, 5 μm)
Mobile phase Methanol (A)-0.1% phosphoric acid aqueous solution (B)
0–10 min, 5–50%A, 95–50%B
10–30 min, 50–50%A, 50–50%B
30–40 min, 50–100%A, 50–0%B
40–55 min, 100–100%A, 0–0%
Flow rate 0.8 mL/min
Column temperature 30 °C
Detection wavelength 270 nm
Sample volume 10 μL