Table 2 Intra-day and inter-day precision and accuracy for the determination of metformin and canagliflozin in human plasma
Studied drug | QC level | Intra-day, n = 6 | Inter-day, n = 6 × 3 | ||||
|---|---|---|---|---|---|---|---|
Mean conc. found (ng/ml) | Accuracy% | RSD% | Mean conc. found (ng/ml) | Accuracy% | RSD% | ||
Metformin | LLOQ (50 ng/mL) | 47.24 | 94.48 | 7.301 | 48.55 | 97.10 | 8.275 |
LQC (150 ng/mL) | 152.17 | 101.45 | 2.980 | 161.15 | 107.43 | 4.980 | |
MQC (1000 ng/mL) | 943.81 | 94.38 | 8.465 | 935.77 | 93.58 | 6.347 | |
HQC (4000 ng/mL) | 3667.95 | 91.70 | 4.114 | 3734.26 | 93.36 | 6.626 | |
Canagliflozin | LLOQ (10 ng/mL) | 11.31 | 113.10 | 1.921 | 11.12 | 111.20 | 4.528 |
LQC (30 ng/mL) | 32.73 | 109.10 | 2.173 | 29.95 | 99.83 | 10.244 | |
MQC (200 ng/mL) | 181.30 | 90.65 | 7.085 | 176.28 | 88.14 | 4.664 | |
HQC (800 ng/mL) | 768.06 | 96.01 | 6.023 | 738.32 | 92.29 | 6.644 | |