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Table 4 Calculation of the system suitability parameters required for testing of TLC and HPLC methods

From: Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Parameter TLC HPLC–UV Reference value
PSE BRM PAR PSE BRM PAR
Retention time (tR)     1.521 2.164 3.414 tR > 1
Retention factor (Rf) 0.24 0.32 0.81     
Column efficiency (N)   2846.3 2042.3 4788.5 N > 2000
Increases with efficiency of the separation
Height equivalent to theoretical plates (HETP)   5.27 × 10−3 7.34 × 10−3 3.13 × 10−3 The smaller the value, the higher the column efficiency
Selectivity factor (α) 1.51 2.48 1.44 1.56 α > 1
Tailing factor (T) 0.786 0.917 0.885 1.13 1.07 1.40 T < 2
T = 1 for symmetric peak
Resolution (Rs) 2.10 8.70 4.13 6.02 Rs > 2