Table 4 Calculation of the system suitability parameters required for testing of TLC and HPLC methods

Retention time (t_R)

1.521

2.164

3.414

t_R > 1

Retention factor (R_f)

Column efficiency (N)

2846.3

2042.3

4788.5

N > 2000

Increases with efficiency of the separation

Height equivalent to theoretical plates (HETP)

5.27 × 10⁻³

7.34 × 10⁻³

3.13 × 10⁻³

The smaller the value, the higher the column efficiency

Selectivity factor (α)

1.44

1.56

α > 1

Tailing factor (T)

1.13

1.07

1.40

T < 2

T = 1 for symmetric peak

Resolution (R_s)

4.13

6.02

R_s > 2