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Table 3 Analysis of PAR, PSE and BRM in Comtrex® maximum strength tablets and application of standard addition technique using the proposed chromatographic methods

From: Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Drug TLC HPLC–UV UPLC-MS/MS Reference methods [26, 29]
Claimed amount taken Added Recovery %a Claimed amount taken Added Recovery %a Claimed amount taken Added Recovery %a,b Recovery %a
PAR
  10.0 (µg/band)
[102.02b]
5.0 101.80 25.0 (µg/mL)
[99.83b]
10.0 100.60 1000.0 (ng/mL)   101.33 102.16
100.22
102.80
103.00
10.0 102.00 25.0 101.04 101.89
14.0 99.20 50.0 101.98 102.07
Mean ± SD 100.97 ± 1.56 Mean ± SD 101.20 ± 0.70 Mean ± SD 101.76 ± 0.386 Mean ± SD
102.55 ± 0.419
Student’s t test 2.52 (2.57)
F-test 1.180 (9.550)
PSE
  3.0 (µg/band)
[100.62b]
1.0 100.00 51.5 (µg/mL)
[98.65b]
30.0 98.87 60.0 (ng/mL)
[98.67b]
30.0 101.60 101.35
101.58
101.58
101.57
3.0 98.00 51.5 98.62 60.0 101.85
10.0 97.60 100.0 97.41 200.0 101.79
Mean ± SD 98.50 ± 1.20 Mean ± SD 98.30 ± 0.78 Mean ± SD 101.75 ± 0.131 Mean ± SD
101.58 ± 0.006
Student’s t-test 2.46 (2.57)
F-test 1.320 (9.550)
BRM
  0.2 (µg/band)
[100.59b]
0.1 100.00 50.1 (µg/mL)
[99.01b]
30.0 100.33 4.0 (ng/mL)
[99.80b]
2.0 101.11 101.15
101.12
101.10
100.13
0.2 100.00 50.1 99.84 4.0 101.16
1.0 99.00 100.0 98.67 100.0 101.17
Mean ± SD 99.67 ± 0.58 Mean ± SD 99.61 ± 0.85 Mean ± SD 101.15 ± 0.032 Mean ± SD
101.13 ± 0.021
Student’s t-test 1.09 (2.57)
F-test 2.390 (9.550)
  1. aAverage of three experiments
  2. bRecovery of the claimed amount taken. Figures between parentheses represent the corresponding tabulated values of t and F at P = 0.05. Reported method for determination of PAR and PSE is an HPLC method using C18 column, a mobile phase composed of 25 mM phosphate buffer (pH = 5):methanol:acetonitrile (30:60:10, v/v/v) at flow rate 1 mL/min and detection at 240 nm. Reported method for determination of BRM is a TLC using methanol:ammonia (100:1.5 v/v) as mobile phase