Table 3 Analysis of PAR, PSE and BRM in Comtrex® maximum strength tablets and application of standard addition technique using the proposed chromatographic methods
Drug | TLC | HPLC–UV | UPLC-MS/MS | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
Claimed amount taken | Added | Recovery %a | Claimed amount taken | Added | Recovery %a | Claimed amount taken | Added | Recovery %a,b | Recovery %a | |
PAR | ||||||||||
10.0 (µg/band) [102.02b] | 5.0 | 101.80 | 25.0 (µg/mL) [99.83b] | 10.0 | 100.60 | 1000.0 (ng/mL) | 101.33 | 102.16 100.22 102.80 103.00 | ||
10.0 | 102.00 | 25.0 | 101.04 | 101.89 | ||||||
14.0 | 99.20 | 50.0 | 101.98 | 102.07 | ||||||
Mean ± SD | 100.97 ± 1.56 | Mean ± SD | 101.20 ± 0.70 | Mean ± SD | 101.76 ± 0.386 | Mean ± SD 102.55 ± 0.419 | ||||
Student’s t test | 2.52 (2.57) | |||||||||
F-test | 1.180 (9.550) | |||||||||
PSE | ||||||||||
3.0 (µg/band) [100.62b] | 1.0 | 100.00 | 51.5 (µg/mL) [98.65b] | 30.0 | 98.87 | 60.0 (ng/mL) [98.67b] | 30.0 | 101.60 | 101.35 101.58 101.58 101.57 | |
3.0 | 98.00 | 51.5 | 98.62 | 60.0 | 101.85 | |||||
10.0 | 97.60 | 100.0 | 97.41 | 200.0 | 101.79 | |||||
Mean ± SD | 98.50 ± 1.20 | Mean ± SD | 98.30 ± 0.78 | Mean ± SD | 101.75 ± 0.131 | Mean ± SD 101.58 ± 0.006 | ||||
Student’s t-test | 2.46 (2.57) | |||||||||
F-test | 1.320 (9.550) | |||||||||
BRM | ||||||||||
0.2 (µg/band) [100.59b] | 0.1 | 100.00 | 50.1 (µg/mL) [99.01b] | 30.0 | 100.33 | 4.0 (ng/mL) [99.80b] | 2.0 | 101.11 | 101.15 101.12 101.10 100.13 | |
0.2 | 100.00 | 50.1 | 99.84 | 4.0 | 101.16 | |||||
1.0 | 99.00 | 100.0 | 98.67 | 100.0 | 101.17 | |||||
Mean ± SD | 99.67 ± 0.58 | Mean ± SD | 99.61 ± 0.85 | Mean ± SD | 101.15 ± 0.032 | Mean ± SD 101.13 ± 0.021 | ||||
Student’s t-test | 1.09 (2.57) | |||||||||
F-test | 2.390 (9.550) | |||||||||