Mix | TLC | HPLC–UV | UPLC-MS/MS | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Concentration | Recovery %a | Concentration | Recovery %a | Concentration | Recovery %a | |||||||||||||
PAR | PSE | BRM | PAR | PSE | BRM | PAR | PSE | BRM | PAR | PSE | BRM | PAR | PSE | BRM | PAR | PSE | BRM | |
1 | 6.0 | 5.0 | 0.8 | 98.54 | 99.80 | 102.53 | 100.0 | 30.0 | 100.0 | 101.09 | 101.46 | 100.29 | 40.0 | 20.0 | 20.0 | 98.44 | 99.13 | 99.96 |
2 | 2.5 | 10.0 | 0.4 | 98.00 | 99.70 | 99.25 | 50.0 | 100.0 | 50.0 | 99.66 | 101.30 | 101.64 | 400.0 | 50.0 | 50.0 | 100.81 | 98.97 | 101.52 |
3 | 2.5 | 2.5 | 0.2 | 98.80 | 98.80 | 101.00 | 50.0 | 50.0 | 50.0 | 102.55 | 98.53 | 101.99 | 120.0 | 100.0 | 200.0 | 99.93 | 101.20 | 101.99 |
4 | 12.5 | 2.5 | 0.2 | 99.20 | 98.00 | 102.00 | 100.0 | 150.0 | 100.0 | 99.75 | 102.32 | 98.88 | 700.0 | 200.0 | 300.0 | 98.52 | 100.53 | 98.56 |
5b | 24.0 | 1.44 | 0.3c | 101.88 | 100.00 | 102.33d | 50.0 | 33.0c | 30.2c | 100.41 | 99.95 | 99.63 | 1000.0 | 60.0 | 4.0 | 100.10 | 100.45 | 99.80 |
6 | 7.5 | 10.0 | 0.4 | 99.60 | 99.10 | 99.50 | 100.0 | 100.0 | 50.0 | 101.73 | 102.25 | 99.51 | 100.0 | 100.0 | 100.0 | 99.89 | 98.97 | 101.52 |
Mean ± SD | 99.30 ± 1.484 | 99.23 ± 0.755 | 101.03 ± 1.470 | 100.87 ± 1.143 | 100.97 ± 1.470 | 100.33 ± 1.390 | 98.85 ± 0.831 | 100.02 ± 1.001 | 100.56 ± 1.328 |