|
Mix
|
TLC
|
HPLC–UV
|
UPLC-MS/MS
|
|---|
|
Concentration
|
Recovery %a
|
Concentration
|
Recovery %a
|
Concentration
|
Recovery %a
|
|---|
|
PAR
|
PSE
|
BRM
|
PAR
|
PSE
|
BRM
|
PAR
|
PSE
|
BRM
|
PAR
|
PSE
|
BRM
|
PAR
|
PSE
|
BRM
|
PAR
|
PSE
|
BRM
|
|---|
|
1
|
6.0
|
5.0
|
0.8
|
98.54
|
99.80
|
102.53
|
100.0
|
30.0
|
100.0
|
101.09
|
101.46
|
100.29
|
40.0
|
20.0
|
20.0
|
98.44
|
99.13
|
99.96
|
|
2
|
2.5
|
10.0
|
0.4
|
98.00
|
99.70
|
99.25
|
50.0
|
100.0
|
50.0
|
99.66
|
101.30
|
101.64
|
400.0
|
50.0
|
50.0
|
100.81
|
98.97
|
101.52
|
|
3
|
2.5
|
2.5
|
0.2
|
98.80
|
98.80
|
101.00
|
50.0
|
50.0
|
50.0
|
102.55
|
98.53
|
101.99
|
120.0
|
100.0
|
200.0
|
99.93
|
101.20
|
101.99
|
|
4
|
12.5
|
2.5
|
0.2
|
99.20
|
98.00
|
102.00
|
100.0
|
150.0
|
100.0
|
99.75
|
102.32
|
98.88
|
700.0
|
200.0
|
300.0
|
98.52
|
100.53
|
98.56
|
|
5b
|
24.0
|
1.44
|
0.3c
|
101.88
|
100.00
|
102.33d
|
50.0
|
33.0c
|
30.2c
|
100.41
|
99.95
|
99.63
|
1000.0
|
60.0
|
4.0
|
100.10
|
100.45
|
99.80
|
|
6
|
7.5
|
10.0
|
0.4
|
99.60
|
99.10
|
99.50
|
100.0
|
100.0
|
50.0
|
101.73
|
102.25
|
99.51
|
100.0
|
100.0
|
100.0
|
99.89
|
98.97
|
101.52
|
|
Mean ± SD
| |
99.30 ± 1.484
|
99.23 ± 0.755
|
101.03 ± 1.470
| |
100.87 ± 1.143
|
100.97 ± 1.470
|
100.33 ± 1.390
| |
98.85 ± 0.831
|
100.02 ± 1.001
|
100.56 ± 1.328
|
- aAverage of three determinations
- bRatio present in dosage form
- cAmount spiked was 0.204 µg/band of BRM for the TLC method and was 30.00 µg/mL of PSE and BRM for HPLC method
- dAfter spiking and subtraction of the added standard
