Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Table 2 Analysis of laboratory prepared mixtures by the proposed chromatographic methods

Mix	TLC						HPLC–UV						UPLC-MS/MS
	Concentration			Recovery %^a			Concentration			Recovery %^a			Concentration			Recovery %^a
	PAR	PSE	BRM	PAR	PSE	BRM	PAR	PSE	BRM	PAR	PSE	BRM	PAR	PSE	BRM	PAR	PSE	BRM
1	6.0	5.0	0.8	98.54	99.80	102.53	100.0	30.0	100.0	101.09	101.46	100.29	40.0	20.0	20.0	98.44	99.13	99.96
2	2.5	10.0	0.4	98.00	99.70	99.25	50.0	100.0	50.0	99.66	101.30	101.64	400.0	50.0	50.0	100.81	98.97	101.52
3	2.5	2.5	0.2	98.80	98.80	101.00	50.0	50.0	50.0	102.55	98.53	101.99	120.0	100.0	200.0	99.93	101.20	101.99
4	12.5	2.5	0.2	99.20	98.00	102.00	100.0	150.0	100.0	99.75	102.32	98.88	700.0	200.0	300.0	98.52	100.53	98.56
5^b	24.0	1.44	0.3^c	101.88	100.00	102.33d	50.0	33.0^c	30.2^c	100.41	99.95	99.63	1000.0	60.0	4.0	100.10	100.45	99.80
6	7.5	10.0	0.4	99.60	99.10	99.50	100.0	100.0	50.0	101.73	102.25	99.51	100.0	100.0	100.0	99.89	98.97	101.52
Mean ± SD				99.30 ± 1.484	99.23 ± 0.755	101.03 ± 1.470				100.87 ± 1.143	100.97 ± 1.470	100.33 ± 1.390				98.85 ± 0.831	100.02 ± 1.001	100.56 ± 1.328

^aAverage of three determinations
^bRatio present in dosage form
^cAmount spiked was 0.204 µg/band of BRM for the TLC method and was 30.00 µg/mL of PSE and BRM for HPLC method
^dAfter spiking and subtraction of the added standard