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Table 1 Regression and validation parameters of the proposed chromatographic methods for determination of PAR, PSE and BRM

From: Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Parameters TLC HPLC–UV UPLC-MS/MS
PAR PSE BRM PAR PSE BRM PAR PSE BRM
Linearity range 2.5–24.0 µg/band 1.0–22.5 µg/band 0.2–15.0 µg/band 5.0–100.0 µg/mL 30.0–200.0 µg/mL 30.0–200.0 µg/mL 40.0–1000.0 ng/mL 6.0–500.0 ng/mL 4.0–500.0 ng/mL
Slope 0.0386 0.0515 0.8877 0.1178 0.03150 0.0364 0.03896 0.1133 0.0030
Intercept 0.5351 0.3440 0.1344 0.4580 − 0.01384 − 0.0039 − 0.9660 2.0740 1.0370
Sy/x 0.0062 0.0043 0.0074 0.0319 0.0546 0.0684 0.1182 0.0202 0.0004
Correlation coefficient (r) 0.9999 1.0000 1.0000 1.000 0.9998 0.9997 0.9997 1.0000 1.0000
Meana 99.46 99.74 100.02 100.67 99.02 100.32 99.55 98.99 98.28
SD 1.57 1.09 0.74 1.43 1.17 1.52 1.27 1.11 0.91
LOD 0.53 0.28 0.03 0.893 5.72 6.21 10.01 0.59 0.44
LOQ 1.61 0.84 0.08 2.71 17.33 18.79 30.34 1.78 1.33
Precision (RSD %)ab 0.36 0.45 0.40 0.31 0.29 0.58 0.27 0.36 0.33
Repeatability
Intermediate precision
1.08 0.99 0.93 0.83 1.15 1.19 1.03 1.53 1.48
Robustness 1.68 1.52 1.26 1.25 1.55 1.67 1.34 0.13 0.03
  1. aAverage of three experiments
  2. bRelative standard deviations (RSD%) of three concentrations, the concentration were as follows: For TLC; PAR (6.0, 10.0, 18.0 µg/band), PSE (5.0, 10.0, 15.0 µg/band) and BRM (0.8, 5.0, 12.0 µg/band), for HPLC; PAR (10.0, 30.0, 50.0 µg/mL), PSE (50.0, 100.0, 150.0 µg/mL) and BRM (0.80, 100.0, 150.0 µg/mL) and for UPLC-MS/MS; PAR (70.0, 120.0, 200.0 ng/mL), PSE (20.0, 50.0, 200.0 ng/mL) and BRM (20.0, 40.0, 100.0 ng/mL)