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Table 1 Regression and validation parameters of the proposed chromatographic methods for determination of PAR, PSE and BRM

From: Analytical methods for the determination of paracetamol, pseudoephedrine and brompheniramine in Comtrex tablets

Parameters

TLC

HPLC–UV

UPLC-MS/MS

PAR

PSE

BRM

PAR

PSE

BRM

PAR

PSE

BRM

Linearity range

2.5–24.0 µg/band

1.0–22.5 µg/band

0.2–15.0 µg/band

5.0–100.0 µg/mL

30.0–200.0 µg/mL

30.0–200.0 µg/mL

40.0–1000.0 ng/mL

6.0–500.0 ng/mL

4.0–500.0 ng/mL

Slope

0.0386

0.0515

0.8877

0.1178

0.03150

0.0364

0.03896

0.1133

0.0030

Intercept

0.5351

0.3440

0.1344

0.4580

− 0.01384

− 0.0039

− 0.9660

2.0740

1.0370

Sy/x

0.0062

0.0043

0.0074

0.0319

0.0546

0.0684

0.1182

0.0202

0.0004

Correlation coefficient (r)

0.9999

1.0000

1.0000

1.000

0.9998

0.9997

0.9997

1.0000

1.0000

Meana

99.46

99.74

100.02

100.67

99.02

100.32

99.55

98.99

98.28

SD

1.57

1.09

0.74

1.43

1.17

1.52

1.27

1.11

0.91

LOD

0.53

0.28

0.03

0.893

5.72

6.21

10.01

0.59

0.44

LOQ

1.61

0.84

0.08

2.71

17.33

18.79

30.34

1.78

1.33

Precision (RSD %)ab

0.36

0.45

0.40

0.31

0.29

0.58

0.27

0.36

0.33

Repeatability

Intermediate precision

1.08

0.99

0.93

0.83

1.15

1.19

1.03

1.53

1.48

Robustness

1.68

1.52

1.26

1.25

1.55

1.67

1.34

0.13

0.03

  1. aAverage of three experiments
  2. bRelative standard deviations (RSD%) of three concentrations, the concentration were as follows: For TLC; PAR (6.0, 10.0, 18.0 µg/band), PSE (5.0, 10.0, 15.0 µg/band) and BRM (0.8, 5.0, 12.0 µg/band), for HPLC; PAR (10.0, 30.0, 50.0 µg/mL), PSE (50.0, 100.0, 150.0 µg/mL) and BRM (0.80, 100.0, 150.0 µg/mL) and for UPLC-MS/MS; PAR (70.0, 120.0, 200.0 ng/mL), PSE (20.0, 50.0, 200.0 ng/mL) and BRM (20.0, 40.0, 100.0 ng/mL)