Parameters | TLC | HPLC–UV | UPLC-MS/MS | ||||||
---|---|---|---|---|---|---|---|---|---|
PAR | PSE | BRM | PAR | PSE | BRM | PAR | PSE | BRM | |
Linearity range | 2.5–24.0 µg/band | 1.0–22.5 µg/band | 0.2–15.0 µg/band | 5.0–100.0 µg/mL | 30.0–200.0 µg/mL | 30.0–200.0 µg/mL | 40.0–1000.0 ng/mL | 6.0–500.0 ng/mL | 4.0–500.0 ng/mL |
Slope | 0.0386 | 0.0515 | 0.8877 | 0.1178 | 0.03150 | 0.0364 | 0.03896 | 0.1133 | 0.0030 |
Intercept | 0.5351 | 0.3440 | 0.1344 | 0.4580 | − 0.01384 | − 0.0039 | − 0.9660 | 2.0740 | 1.0370 |
Sy/x | 0.0062 | 0.0043 | 0.0074 | 0.0319 | 0.0546 | 0.0684 | 0.1182 | 0.0202 | 0.0004 |
Correlation coefficient (r) | 0.9999 | 1.0000 | 1.0000 | 1.000 | 0.9998 | 0.9997 | 0.9997 | 1.0000 | 1.0000 |
Meana | 99.46 | 99.74 | 100.02 | 100.67 | 99.02 | 100.32 | 99.55 | 98.99 | 98.28 |
SD | 1.57 | 1.09 | 0.74 | 1.43 | 1.17 | 1.52 | 1.27 | 1.11 | 0.91 |
LOD | 0.53 | 0.28 | 0.03 | 0.893 | 5.72 | 6.21 | 10.01 | 0.59 | 0.44 |
LOQ | 1.61 | 0.84 | 0.08 | 2.71 | 17.33 | 18.79 | 30.34 | 1.78 | 1.33 |
Precision (RSD %)ab | 0.36 | 0.45 | 0.40 | 0.31 | 0.29 | 0.58 | 0.27 | 0.36 | 0.33 |
Repeatability Intermediate precision | 1.08 | 0.99 | 0.93 | 0.83 | 1.15 | 1.19 | 1.03 | 1.53 | 1.48 |
Robustness | 1.68 | 1.52 | 1.26 | 1.25 | 1.55 | 1.67 | 1.34 | 0.13 | 0.03 |