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Table 4 Assay results for the determination of the FF and GLY in their synthetic mixture and MDI by the proposed DS and reference methods

From: Multiple analytical methods for determination of formoterol and glycopyrronium simultaneously in their novel combined metered dose inhaler

  a. Proposed DS method (synthetic mixture) Reference method [39]
Conc. taken (μg/mL) % Founda Conc. taken (μg/mL) % Founda
FF GLY FF GLY FF GLY FF GLY
Data 8.4 15.75 100.08 100.12 10.0 18.0 98.24. 99.58
7.2 13.5 99.48 100.82 8.4 15.0 99.42 98.35
6 11.25 99.56 100.40 7.8 12.0 100.13 101.35
4.8 9 100.21 100.35 6.2 9.0 99.25 100.89
3.6 6.75 100.03 99.49 4.5 6.0 100.47 99.36
2.4 4.5 99.99 99.18 2.9 3.0 100.34 100.21
\({\bar{\text{X}}}\)   99.89 100.06   99.92 99.96
± SD 0.29 0.61 0.55 1.1
t-value 0.32(10.0) 0.20 (10.0)  
F-value 3.62 (5.5) 3.16 (5.5)
  1. Nominal content for GLY in Bevespi® aerosphere by the proposed method = 9.0 μg/buff
  2. Each result is the average of three separate determinations
  3. aNominal content for FF in Bevespi® aerosphere by the proposed method = 4.8 μg/buff
  b. Proposed DS method (MDI) Reference method [39]
Conc. taken (μg/mL) % Founda Conc. taken (μg/mL) % Founda
FF GLY FF GLY FF GLY FF GLY
Data 8.4 15.75 100.89 100.88 10.0 18.0 99.21 100.28
7.2 13.5 99.52 100.25 8.4 15.0 98.90 99.35
6 11.25 100.35 100.54 7.8 12.0 100.51 98.52
4.8 9 99.87 99.35 6.2 9.0 99.54 101.35
3.6 6.75 100.35 100.48 4.5 6.0 100.37 100.47
2.4 4.5 100.08 99.36 2.9 3.0 98.50 98.52
\({\bar{\text{X}}}\)   100.18 100.14   99.51 99.75
± SD 0.47 0.67 0.33 1.14
t-value 1.41 (10.0) 0.74 (10.0)  
F-value 3.24 (5.5) 3.17 (5.5)
  1. Nominal content for GLY in Bevespi® aerosphere by the proposed method = 9.0 μg/buff
  2. Each result is the average of three separate determinations
  3. aNominal content for FF in Bevespi® aerosphere by the proposed method = 4.8 μg/buff