Multiple analytical methods for determination of formoterol and glycopyrronium simultaneously in their novel combined metered dose inhaler

Table 3 Precision data of determination of the FF and GLY in pure form using (A) spectrophotometric method and (B) IPC method

(A) Spectrophotometric method
Derivative spectrophotometric method	FF Conc_208.27nm (μg/mL)			GLY Conc_239.6nm (μg/mL)
Derivative spectrophotometric method	4.8	6.0	7.2	6.75	9.0	11.25
Intra-day
\({\bar{\text{X}}}\)	100.14	99.80	100.03	100.13	99.21	100.33
± SD	0.29	0.61	0.68	0.29	0.20	0.75
% RSD	0.29	0.61	0.68	0.29	0.20	0.75
% Error	0.17	0.35	0.39	0.17	0.12	0.43
Inter-day
\({\bar{\text{X}}}\)	100.12	100.24	99.86	100.14	99.60	100.12
± SD	0.21	0.52	0.95	0.75	0.42	0.55
% RSD	0.21	0.52	0.95	0.74	0.42	0.55
% Error	0.12	0.30	0.55	0.43	0.24	0.32

Ratio derivative spectrophotometric method	FF Conc_214/229nm (μg/mL)			GLY Conc_240/259nm (μg/mL)
Ratio derivative spectrophotometric method	4.8	6.0	7.2	6.75	9.0	11.25
Intra-day
\({\bar{\text{X}}}\)	99.89	100.07	99.82	100.64	100.74	99.91
± SD	0.58	0.85	0.49	0.36	0.29	0.64
% RSD	0.58	0.85	0.49	0.36	0.29	0.64
% Error	0.33	0.49	0.28	0.21	0.17	0.37
Inter-day
\({\bar{\text{X}}}\)	100.53	100.08	100.43	99.82	99.44	100.47
± SD	0.23	0.54	0.75	0.96	0.13	0.76
% RSD	0.23	0.54	0.74	0.96	0.13	0.75
% Error	0.13	0.31	0.43	0.55	0.07	0.44

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