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Table 1 Intra-and inter-day assay precision and accuracy for the determination of 5-HT in RPMI medium 1640

From: A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

ASSAY Nominal concentration (μg/mL) Mean measured concentration (μg/mL) CV (%) Accuracy (%)
Intra‐assay (n = 5) 0.1 0.093 2.45 92.96
0.3 0.305 1.12 101.71
4 4.060 1.25 101.50
7.5 7.493 0.32 99.90
Inter‐assay (n = 15) 0.1 0.092 4.63 92.02
0.3 0.310 3.82 103.28
4 3.981 4.69 99.53
7.5 7.518 0.62 100.24
  1. Intra-and inter-day assay precision and accuracy for the determination of 5-HT in RPMI medium 1640