Table 7 Quantification of different protein and biopharmaceutical samples with the use of well recovered amino acids
From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis
| BSA | KLH batch no. 1 | KLH batch no. 2 | KLH batch no. 3 | Enbrel | |
|---|---|---|---|---|---|
| Alanine | 4.88 | 19.85 | 20.36 | 20.76 | 48.74 |
| Leucine | 4.86 | 19.00 | 21.09 | 20.57 | 45.85 |
| Asparagine | 4.32 | 19.84 | 21.35 | 19.27 | 49.77 |
| Aspartic acid | |||||
| Glutamine | 5.25 | 20.09 | 19.98 | 20.13 | 49.45 |
| Glutamic acid | |||||
| Phenylalanine | 4.33 | 21.37 | 18.92 | 19.93 | 53.74 |
| Arginine | 4.71 | 20.26 | 18.27 | 19.55 | – |
| Tyrosine | 5.48 | 20.37 | 20.09 | 19.71 | 49.54 |
| Determined concentration | 4.83 ± 0.43 mg mL−1 | 20.11 ± 0.71 mg mL−1 | 20.01 ± 1.10 mg mL−1 | 19.99 ± 0.54 mg mL−1 | 49.52 ± 2.53 mg mL−1 |
| Expected concentration | 5 mg mL−1 | 20 mg mL−1 | 20 mg mL−1 | 20 mg mL−1 | 50 mg mL−1 |