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Table 7 Quantification of different protein and biopharmaceutical samples with the use of well recovered amino acids

From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis

  BSA KLH batch no. 1 KLH batch no. 2 KLH batch no. 3 Enbrel
Alanine 4.88 19.85 20.36 20.76 48.74
Leucine 4.86 19.00 21.09 20.57 45.85
Asparagine 4.32 19.84 21.35 19.27 49.77
Aspartic acid
Glutamine 5.25 20.09 19.98 20.13 49.45
Glutamic acid
Phenylalanine 4.33 21.37 18.92 19.93 53.74
Arginine 4.71 20.26 18.27 19.55
Tyrosine 5.48 20.37 20.09 19.71 49.54
Determined concentration 4.83 ± 0.43 mg mL−1 20.11 ± 0.71 mg mL−1 20.01 ± 1.10 mg mL−1 19.99 ± 0.54 mg mL−1 49.52 ± 2.53 mg mL−1
Expected concentration 5 mg mL−1 20 mg mL−1 20 mg mL−1 20 mg mL−1 50 mg mL−1